Glyphosate-induced stiffening of HaCaT keratinocytes, a Peak Force Tapping study on living cells
Glyphosate-induced stiffening of HaCaT keratinocytes, a Peak Force Tapping study on living cells
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DOI：
10.1016/j.jsb.2012.02.007
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发表时间：
2012-04-01
影响因子：
3
通讯作者：
Nicod, Laurence
中科院分区：
文献类型：
--
作者：
Heu, Celine;Berquand, Alexandre;Nicod, Laurence
皮肤是第一道生理屏障，具有复杂的结构，提供抵御多种物理和化学侵害的防御功能。草甘膦是一种广泛使用的除草剂，已被证明会增加患癌症的风险。此外，越来越多的证据表明机械表型在恶性转化中起重要作用。原子力显微镜（AFM）在过去的十年中已经成为一个强大的工具，提供了一个纳米尺度的分辨率成像的生物样品。峰值力攻丝（PFT）是一种新发布的基于AFM的调查技术，允许以相对较高的速度和高分辨率从各种样品中提取化学和机械性能。本工作使用PET技术来研究HaCaT角质形成细胞（一种人类表皮细胞系），并提供了一种新颖的方法来研究在接近生理条件下化学诱导的细胞机械特性变化。这些实验表明，草甘膦诱导细胞膜硬化，并在亚细胞水平上的细胞骨架结构的外观，对于低细胞毒性浓度，而暴露于IC 50（抑制浓度50%）处理的细胞表现出控制一样的机械行为，尽管明显的膜损伤。槲皮素，一种众所周知的抗氧化剂，逆转草甘膦诱导的机械表型。(C)2012 Elsevier Inc. All rights reserved.
The skin is the first physiological barrier, with a complex constitution, that provides defensive functions against multiple physical and chemical aggressions. Glyphosate is an extensively used herbicide that has been shown to increase the risk of cancer. Moreover there is increasing evidence suggesting that the mechanical phenotype plays an important role in malignant transformation. Atomic force microscopy (AFM) has emerged within the last decade as a powerful tool for providing a nanometer-scale resolution imaging of biological samples. Peak Force Tapping (PFT) is a newly released AFM-based investigation technique allowing extraction of chemical and mechanical properties from a wide range of samples at a relatively high speed and a high resolution. The present work uses the PET technology to investigate HaCaT keratinocytes, a human epidermal cell line, and offers an original approach to study chemically-induced changes in the cellular mechanical properties under near-physiological conditions. These experiments indicate glyphosate induces cell membrane stiffening, and the appearance of cytoskeleton structures at a subcellular level, for low cytotoxic concentrations whereas cells exposed to IC50 (inhibitory concentration 50%) treatment exhibit control-like mechanical behavior despite obvious membrane damages. Quercetin, a well-known antioxidant, reverses the glyphosate-induced mechanical phenotype. (C) 2012 Elsevier Inc. All rights reserved.