DNA extraction from olive oil and its use in the identification of the production cultivar
DNA extraction from olive oil and its use in the identification of the production cultivar
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DOI：
10.1016/s0308-8146(03)00218-8
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发表时间：
2003-10-01
期刊：
影响因子：
8.8
通讯作者：
Fogher, C
中科院分区：
文献类型：
--
作者：
Busconi, M;Foroni, C;Fogher, C
当必须追踪生产过程中使用的原材料时，从食品样品中回收DNA可能非常重要。我们有兴趣验证特级初榨橄榄油中是否存在核酸，以确定用于生产的橄榄的原产品种。一种可靠的特级初榨橄榄油DNA提取方法已经确定，就数量和质量而言，并证明了使用这种DNA对原始品种进行指纹鉴定的可能性。DNA提取进行了测试，对四个单一品种的油，加上四个商业特级初榨橄榄油。提取液中的DNA是叶绿体和核的来源，因为我们能够扩增克隆品种的RAPD和AFLP片段同源的其他物种的核DNA。还表明，从油中纯化的DNA可用于AFLP分析，并且从单品种油中纯化的DNA的谱对应于从同一栽培品种的叶中纯化的DNA的谱。(C)2003爱思唯尔有限公司。保留所有权利。
DNA recovery from food samples might be of great importance when the raw material used in the production process has to be traced. We were interested in verifying the presence of nucleic acids in extra virgin olive oil in order to determine the cultivar of origin of the olives used for the production. A reliable DNA extraction method for extra virgin olive oil has been defined, as far as both quantity and quality are concerned, and the possibility of using this DNA for fingerprinting the original cultivar has been demonstrated. DNA extraction was tested on four monovariety oils, plus four commercial extra virgin olive oils. The DNA in the extracted solution was of chloroplast and nuclear origin since we were able to amplify cloned cultivar RAPD and AFLP fragments homologous to nuclear DNA of other species. It has also been shown that DNA purified from oil can be used for AFLP analysis and that the profile of the DNA purified from a monovariety oil corresponds to the profile of the DNA purified from the leaves of the same cultivar. (C) 2003 Elsevier Ltd. All rights reserved.