A BLOOD-GROUP SDA-ACTIVE PENTASACCHARIDE ISOLATED FROM TAMM-HORSFALL URINARY GLYCOPROTEIN
A BLOOD-GROUP SDA-ACTIVE PENTASACCHARIDE ISOLATED FROM TAMM-HORSFALL URINARY GLYCOPROTEIN
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DOI：
10.1016/s0006-291x(83)80190-9
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
WATKINS, WM
中科院分区：
文献类型：
--
作者：
DONALD, ASR;YATES, AD;WATKINS, WM
用半乳糖氧化酶和硼氢化钠处理SD(a+)血型人的Tamm-Horsfall尿糖蛋白，然后用内切-β-半乳糖苷酶消化。释放了一系列可透析的标记片段，从中分离出一种五糖，它能强烈抑制人抗SDA血清与SD(a+)红细胞的凝集，因此含有SDA决定簇结构。还原、甲基化分析和顺序的胞外糖苷酶消化确定了五糖的结构为：GalNAc.beta(1.fwdarw.4)[NeuAc(2.fwdarw.3)[Gal.Beta.(1.fwdarw.4)GlcNAc.beta(1.fwdarw.3)Gal.
Human Tamm-Horsfall urinary glycoprotein from an individual of the blood group Sd(a+) phenotype was 3H-labeled by treatment with galactose oxidase and sodium boro[3H]hydride and was then digested with endo-.beta.-galactosidase. A series of dialyzable, labeled fragments was released from which a pentasaccharide was isolated that strongly inhibited the agglutination of Sd(a+) red cells by human anti-Sda serum and hence contained the Sda determinant structure. Reduction, methylation analysis and sequential exo-glycosidase digestion established the structure of the pentasaccharide as: GalNAc.beta.(1 .fwdarw. 4)[NeuAc(2 .fwdarw. 3)]Gal.beta.(1 .fwdarw. 4)GlcNAc.beta.(1 .fwdarw. 3)Gal.