Serum- and Glucocorticoid-Regulated Kinase 1 Is Required for Nuclear Export of the Ribonucleoprotein of Influenza A Virus
Serum- and Glucocorticoid-Regulated Kinase 1 Is Required for Nuclear Export of the Ribonucleoprotein of Influenza A Virus
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DOI：
10.1128/jvi.01258-12
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发表时间：
2013-05-01
影响因子：
5.4
通讯作者：
Palese, Peter
中科院分区：
文献类型：
--
作者：
Alamares-Sapuay, Judith G.;Martinez-Gil, Luis;Palese, Peter
我们先前进行了小干扰RNA（siRNA）筛选，并确定血清和糖皮质激素调节激酶1（SGK1）作为甲型流感病毒复制所需的宿主因子。然而，SGK1在流感病毒生命周期中的作用从未被研究过。在这项研究中，我们证明了SGK1是流感病毒最佳复制所必需的，使用SGK1抑制剂GSK 650394和SGK1特异性siRNA。我们还表明，SGK1是所需的病毒核糖核蛋白核出口。
We previously performed a small interfering RNA (siRNA) screen and identified serum-and glucocorticoid-regulated kinase 1 (SGK1) as a host factor required for influenza A virus replication. However, the role of SGK1 in the influenza viral life cycle has never been examined. In this study, we demonstrate that SGK1 is required for optimal replication of influenza virus, using the SGK1 inhibitor GSK 650394 and SGK1-specific siRNAs. We also demonstrate that SGK1 is required for viral ribonucleoprotein nuclear export.