A transient CD15 immunoreactive sling in the developing mouse cerebellum
A transient CD15 immunoreactive sling in the developing mouse cerebellum
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DOI：
10.1016/s0736-5748(97)00036-1
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发表时间：
1997-11-01
影响因子：
1.8
通讯作者：
Mai, JK
中科院分区：
文献类型：
--
作者：
Ashwell, KWS;Mai, JK
用石蜡切片免疫组织化学方法研究了3-岩藻糖基-N-乙酰乳糖胺（FAL，CD 15）抗原表位在发育中小鼠小脑的分布。CD 15免疫反应性首先出现在E15作为一个离散的束的过程躺在下面，并稍微内，更深的层的外部颗粒层。到E17时，小脑原基已完成中线融合。从小脑原基外侧界的小脑后中线周围的小脑三角区到对侧的小脑三角区，可追踪到这些区域。通过PZ，这种吊索不再明显，CD 15免疫反应性仅限于小脑白色物质中的星形胶质细胞。CD 15免疫反应性过程通过发育中的小脑追求不寻常的轨迹，这与小脑中任何其他先前描述的轴突或神经胶质过程束不同。从它的轨迹和与脑室表面的关联，似乎这个结构，我们已经命名为横向小脑吊索，是由胶质细胞的过程，虽然它是不免疫反应的S-100或胶质细胞酸性蛋白。这一短暂的外观吊索环绕小脑后部提示在产前小脑形态发生的作用。(C)1997年ISDN。出版社：Elsevier Science Ltd
The distribution of the 3-fucosyl-N-acetyl-lactosamine (FAL, CD15) epitope in the developing mouse cerebellum was examined with the aid of immunohistochemistry of paraffin sections. CD15 immunoreactivity first appeared at E15 as a discrete bundle of processes lying beneath, and slightly within, the deeper lavers of the external granular layer. By E17, when the cerebellar anlagen had completed their midline fusion. these professes could be traced from the germinal trigone at the lateral limits of the celebellar anlage around the posterior cerebellar midline to the opposite germinal trigone. By PZ, this sling was no longer apparent and CD15 immunoreactivity was confined to astrocytes in the cerebellar white matter. surrounding the deep cerebellar nuclei.The CD15 immunoreactive processes pursue an unusual trajectory through the developing cerebellum unlike any other previously described axonal or glial process bundle in the cerebellum. From its trajectory and association with the ventricular surface it seems that this structure, which we have named the transverse cerebellar sling, is composed of glial processes, although it was not immunoreactive for S-100 or glial fibrillary acidic protein. The transient appearance of this sling encircling the posterior cerebellum is suggestive of a role in prenatal cerebellar morphogenesis. (C) 1997 ISDN. published by Elsevier Science Ltd.