The expression of B cell surface receptors. III. The murine low-affinity IgE Fc receptor is not expressed on Ly 1 or 'Ly 1-like' B cells.
The expression of B cell surface receptors. III. The murine low-affinity IgE Fc receptor is not expressed on Ly 1 or 'Ly 1-like' B cells.
复制标题
B 细胞表面受体的表达。
DOI：
10.1093/intimm/3.4.305
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发表时间：
1991
影响因子：
4.4
通讯作者：
Lynch,RG
中科院分区：
文献类型：
--
作者：
Waldschmidt,TJ;Kroese,FG;Tygrett,LT;Conrad,DH;Lynch,RG
在正常成年小鼠中检查了 IgE FcR (FcεR) 阳性和阴性 B 细胞的分布。使用三色流式细胞术，分析腹膜和脾脏中存在的各种 B 细胞亚群的 FcεR 表达。结果表明，在腹膜腔中，FcεR 不在大多数 Ly 1+B 细胞和 Ly 1−, Mac 1+ 姐妹 B 细胞上表达。然而，该受体存在于腹膜中的少数常规 B 细胞上。尽管白细胞介素 4 (IL-4) 可以增加常规 B 细胞上 FcεR 的水平，但将 Ly 1 和姐妹 B 细胞与 IL-4 一起孵育不会导致 FcεR 的表达。当检查脾脏中存在的 B 细胞时，一小部分 B 细胞始终被发现是 FcεR-。这些 FcεR- 细胞 IgM 亮，IgD 暗，大部分 Ly 1 和 Mac 1 阴性。精神组织切片染色显示，FcεR-B 细胞主要位于边缘区，而 FcεR+B 细胞则发现于毛囊中。综上所述，结果表明 FcεR 可能是描绘各种 B 细胞亚群的有用标记。在腹膜中，FcεR 似乎能够将常规 B 细胞与 Ly 1/姐妹谱系的 B 细胞区分开来，而在脾脏中，FcεR 可能会将静息滤泡 B 细胞与 Ly 1/姐妹和边缘区 B 细胞区分开来。
The distribution of IgE FcR (FcεR)-positive and -negative B celIs was examined in normal adult mice. Using three-color flow cytometry, the expression of the FcεR was analyzed on various B-cell subsets present in the peritoneum and spleen. The results demonstrate that in the peritoneal cavity, the FcεR is not expressed on the large majority of Ly 1+B cells and Ly 1−, Mac 1+sister B cells, The receptor is present, however, on the small number of conventional B cells residing in the peritoneum. Although interleukin 4 (IL-4) can increase the levels of the FcεR on conventional B cells, incubation of Ly 1 and sister B cells with IL-4 did not result in the expression of the FcεR. When examining B cells present in the spleen, a small subset of B cells was consistently found to be FcεR-. These FcεR- cells were IgM-bright, IgD-dull and largely Ly 1- and Mac 1-negative. Staining of spienic tissue sections revealed that the FcεR- B cells were primarily localized to the marginal zones, whereas the FcεR+B cells were found in the follicies. Taken together, the results indicate that the FcεR may be a useful marker in delineating the various B-cell subsets. In the peritoneum, the FcεR appears to discriminate conventional B cells from those of the Ly 1/sister lineage, and in the spleen it is likely to distinguish resting follicular B cells from Ly 1/sister and marginal zone B cells.