The expression of receptor for advanced glycation end products is associated with angiogenesis in human oral squamous cell carcinoma
The expression of receptor for advanced glycation end products is associated with angiogenesis in human oral squamous cell carcinoma
复制标题
DOI：
10.1007/s00428-006-0359-2
复制
发表时间：
2007-03-01
期刊：
影响因子：
3.5
通讯作者：
Kuniyasu, Hiroki
中科院分区：
文献类型：
--
作者：
Sasahira, Tomonori;Kirita, Tadaaki;Kuniyasu, Hiroki
晚期糖基化终末产物受体（receptor for advanced glycation end products，简称AGEs）与多种人类癌症的进展相关。在这项研究中，我们研究了在口腔鳞状细胞癌（OSCC）血管生成的作用。采用酶联免疫吸附试验（ELISA）检测20例口腔鳞癌组织中的β-内酰胺酶浓度。免疫组化法检测微血管密度（MVD）和淋巴管密度（LVD）。ELISA法检测肿瘤组织中血管内皮生长因子（VEGF）和VEGF-C的含量。肿瘤组织中VEGF浓度与MVD（P = 0.0123）和VEGF浓度（P = 0.0344）相关，而与LVD和VEGF-C浓度无关。在人OSCC细胞系HSC-3和HSC-4中，用HMGB-1处理可增加VEGF的分泌，但不增加VEGF-C的分泌。HMGB-1的作用被反义S-寡核苷酸下调HMGB-1的表达所消除。这些结果表明，在口腔鳞状细胞癌的血管生成密切相关的表达。
The receptor for advanced glycation end products (RAGE) is associated with cancer progression in several human cancers. In this study, we examined the roles of RAGE in the angiogenesis of oral squamous cell carcinoma (OSCC). RAGE concentration was examined in 20 OSCC tumors by enzyme-linked immunosorbent assay (ELISA). The microvessel density (MVD) and lymph vessel density (LVD) were examined by immunostaining. Concentrations of vascular endothelial growth factor (VEGF) and VEGF-C were examined in tumor tissues by ELISA. Tumoral RAGE concentration was associated with higher tumor MVD (P = 0.0123) and tumor VEGF concentration (P = 0.0344), but not with LVD and VEGF-C concentration. Treatment with RAGE ligand, high-mobility group box (HMGB)-1 increased the secretion of VEGF but not that of VEGF-C in human OSCC cell lines, HSC-3 and HSC-4. The effect of HMGB-1 was abrogated by RAGE down-regulation by antisense S-oligodeoxynucleic acid. These results suggest that RAGE expression is closely associated to angiogenesis in OSCC.