High-voltage capacitively coupled contactless conductivity detection for microchip capillary electrophoresis
High-voltage capacitively coupled contactless conductivity detection for microchip capillary electrophoresis
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DOI：
10.1021/ac020489
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发表时间：
2002-12-15
影响因子：
7.4
通讯作者：
Hauser, PC
中科院分区：
文献类型：
--
作者：
Tanyanyiwa, J;Hauser, PC
使用500 Vp-p的交流激励电压和100 kHz的频率，通过电容耦合在平面电泳装置上进行非接触式电导率检测。可以通过1mm厚的盖板以这种方式进行检测。然而，通过将电极放入允许与分离通道更紧密耦合的槽中，可以获得更好的灵敏度。对于小无机离子 K+、Na+ 和 Mg2+，3 x S/N 检测限为 0.49、0.41 和 0.35 muM。以 Mn2+、Zn2+ 和 Cr3+ 为例演示了重金属的检测，检测限分别为 2.1、2.8 和 6.8 μM。通过检测食品和饮料分析中感兴趣的柠檬酸和乳酸，以及检测三种抗炎非类固醇药物 4-乙酰胺苯酚、布洛芬和水杨酸（作为制药感兴趣的物种的例子），进一步说明了该方法的通用性。
Contactless conductivity detection was carried out on a planar electrophoresis device by capacitive coupling using an ac excitation voltage of 500 Vp-p and a frequency of 100 kHz. It was possible to carry out detection in this way through a cover plate of 1 mm thickness. Better sensitivity is obtained, however, by placing the electrodes into troughs that allow tighter coupling to the separation channel. The 3 x S/N detection limits are 0.49, 0.41, and 0.35 muM for the small inorganic ions K+, Na+, and Mg2+. The detection of heavy metals is demonstrated with the example of Mn2+, Zn2+, and Cr3+ with detection limits of 2.1, 2.8, and 6.8 muM, respectively. The universal nature of the method is further illustrated by the detection of citric and lactic acids, which are of interest in food and beverage analysis, and detection of three antinflammatory nonsteroid drugs, 4-acetamidophenol, ibuprofen, and salicylic acid, as examples of species of pharmaceutical interest.