Desulfoglaeba alkanexedens gen. nov., sp nov., an n-alkane-degrading, sulfate-reducing bacterium
Desulfoglaeba alkanexedens gen. nov., sp nov., an n-alkane-degrading, sulfate-reducing bacterium
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DOI：
10.1099/ijs.0.64398-0
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发表时间：
2006-12-01
影响因子：
2.8
通讯作者：
Suflita, Joseph M.
中科院分区：
文献类型：
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作者：
Davidova, Irene A.;Duncan, Kathleen E.;Suflita, Joseph M.
从美国弗吉尼亚州海军含油废水储存设施和美国俄克拉何马州油田采出水中分离到两株能够氧化正构烷烃的新型硫酸盐还原细菌ALDC(T)和Lake。模式菌株(ALDC(T))具有较窄的底物专一性，只能与正构烷烃(从C-6到C-12)、丙酮酸、丁酸、己酸和4-甲基辛酸一起生长。ALDC(T)菌株的细胞革兰氏染色阴性，稍弯曲，短杆，末端为椭圆形(2.5-3.0×1.0-1.4微米)，常成对出现。细胞倾向于形成聚集体或大簇，不活动，不形成内孢子。最适生长温度为31-37℃，最适生长pH为6.5-7.2。盐不是生长所必需的，但盐浓度高达55g L(-1)是可以耐受的。DNA G+C含量为53.6mol%。16S rRNA基因的系统发育分析表明，ALDC(T)株与Lake株亲缘关系较近，但并不完全相同(99.9%的相似性)。这两个菌株与其他已知的烷烃降解菌、硫酸盐还原细菌或三角洲蛋白细菌的其他属没有密切的亲缘关系。因此，菌株ALDC(T)(=JCM 13588(T)=ATCC BAA-1302(T))代表了一个新种和属的模式菌株，命名为Desulfoglaeba alkanexedens gen。Nov.，sp.11月
Two novel sulfate-reducing bacteria, strains ALDC(T) and Lake, which were able to oxidize n-alkanes, were isolated from a naval oily wastewater-storage facility (VA, USA) and from oilfield production water (OK, USA), respectively. The type strain (ALDC(T)) had a narrow substrate specificity and could grow only with n-alkanes (from C-6 to C-12), pyruvate, butyrate, hexanoic acid and 4-methyloctanoic acid. Cells of strain ALDC(T) stained Gram-negative and were slightly curved, short rods with oval ends (2.5-3.0 x 1.0-1.4 mu m), often occurring in pairs. Cells tended to form aggregates or large clusters and were non-motile and did not form endospores. Optimum growth occurred between 31 and 37 degrees C and at pH 6.5-7.2. NaCl was not required for growth, but salt concentrations up to 55 g l(-1) could be tolerated. The DNA G + C content was 53.6 mol%. Phylogenetic analysis of the 16S rRNA genes revealed that strains ALDC(T) and Lake were closely related, but not identical (99.9% similarity). The two strains were not closely related to other known alkane-degrading, sulfate-reducing bacteria or to other genera of the Deltaproteobacteria. Therefore, it is proposed that strain ALDC(T) (= JCM 13588(T) = ATCC BAA-1302(T)) represents the type strain of a novel species and genus, with the name Desulfoglaeba alkanexedens gen. nov., sp. nov.