Steinernema brazilense n. sp (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Mato Grosso, Brazil

Steinernema brazilense n. sp (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Mato Grosso, Brazil
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DOI:
10.1016/j.jip.2009.09.004
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发表时间:
2010-01-01
影响因子:
3.4
通讯作者:
Harakava, Ricardo
Harakava, Ricardo
中科院分区:
生物学3区
文献类型:
--
作者:
Nguyen, Khuong B.;Ambros Ginarte, Carmen M.;Harakava, Ricardo

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一种新的昆虫病原线虫,巴西斯氏线虫 n. sp.,是从巴西南马托格罗索州天然森林采集的单个土壤样本中分离出来的。巴西沙门氏菌sp。形态学上具有感染性幼虫(IJ)、雄性和雌性的特征。对于IJ,体长平均1157(1023-1284)μm,从前端到排泄孔的距离95(87-102)μm,从前端到食管末端148(139-153)μm,尾长85(80-104)μm,D%和E%值为63(58-70)和106 (95-118.0),分别。横向场模式变量;脊从头到尾的排列公式为:2、4、6、8、6、2。对于男性,诊断特征包括骨针,平均83(75-89)μm;对于男性,诊断特征包括骨针,平均83(75-89)μm; D%约65;比率SW%约为192。针头的长度大于宽度。侧田有一条狭窄的山脊。第一代雌性的特征是存在腹侧肛后肿胀。巴西沙门氏菌sp。形态上与斯氏线虫很接近。与S.diaprepesi的区别在于其IJ体长较长(1157比1002μm),前端到排泄孔的距离较长(1110比75μm),尾长较长(103比83μm);新物种的雄性具有更长的针状体(83对79μm)。通过ITS和D2D3区域的rDNA特征,可以将新种与格氏斯氏线虫组的其他成员进一步区分。由爱思唯尔公司出版
A new entomopathogenic nematode, Steinernema brazilense n. sp., was isolated from a single soil sample collected from a natural forest in Mato Grosso do Sul state, Brazil. S. brazilense n. sp. is characterized morphologically by features of infective juveniles (IJ), males and females. For the IJ, body length averaging 1157 (1023-1284) mu m, distance from anterior end to excretory pore 95 (87-102) mu m, from anterior end to end of esophagus 148 (139-153) mu m, tail length 85 (80-104) mu m, D% and E% values 63 (58-70) and 106 (95-118.0), respectively. Lateral field pattern variable; the formula for the arrangement of ridges from head to tail is: 2, 4, 6, 8, 6, 2. For the male, the diagnostic characters include spicule averaging 83 (75-89) mu m; D% about 65; the ratio SW% about 192. The length of spicule head is greater than width. Lateral field with one narrow ridge. First generation females are characterized by the presence of a ventral postanal swelling. S. brazilense n. sp. is morphologically close to Steinernema diaprepesi. It can be differentiated from S. diaprepesi by its longer IJ body length (1157 vs 1002 mu m), longer distance from anterior end to excretory pore (1110 vs 75 mu m), a longer tail length (103 vs 83 mu m); males of the new species with longer spicule (83 vs 79 mu m). The new species can be distinguished further from other members of Steinernema glaseri group by characteristics of rDNA of ITS and D2D3 regions. Published by Elsevier Inc.