Comparative genomics of human Lactobacillus crispatus isolates reveals genes for glycosylation and glycogen degradation: implications for in vivo dominance of the vaginal microbiota
Comparative genomics of human Lactobacillus crispatus isolates reveals genes for glycosylation and glycogen degradation: implications for in vivo dominance of the vaginal microbiota
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DOI：
10.1101/441972
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发表时间：
2018-10
期刊：
影响因子：
15.5
通讯作者：
C. van der Veer;R. Hertzberger;S. Bruisten;Hanne L. P. Tytgat;Jorne Swanenburg;Alie de Kat Angelino-Bart;F. Schuren;D. Molenaar;G. Reid;H. D. de Vries;R. Kort
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文献类型：
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作者：
C. van der Veer;R. Hertzberger;S. Bruisten;Hanne L. P. Tytgat;Jorne Swanenburg;Alie de Kat Angelino-Bart;F. Schuren;D. Molenaar;G. Reid;H. D. de Vries;R. Kort
以乳杆菌（特别是卷曲乳杆菌）为主的阴道微生物群与阴道健康相关，而不以乳杆菌为主的阴道微生物群被认为是菌群失调。在这里，我们研究了从具有乳杆菌为主的阴道微生物群（LVM）的女性阴道中分离出的卷曲乳杆菌菌株是否与从具有菌群失调的阴道微生物群（DVM）的阴道样本中分离出的卷曲乳杆菌菌株在表型或基因型上不同。我们研究了 33 个卷曲乳杆菌菌株（n = 16 来自 LVM；n = 17 来自 DVM）。两组菌株比较表明，虽然存在菌株差异，但两组均降解各种碳水化合物，产生相似量的有机酸，抑制淋病奈瑟菌生长，且不产生生物膜。对 28 个菌株（n = 12 LVM；n = 16 DVM）的比较基因组分析揭示了一种新型的 3 片段糖基转移酶基因，该基因在从 DVM 分离的菌株中更为普遍。大多数卷曲乳杆菌菌株在补充糖原的生长培养基上表现出生长。糖原生长效率较低 (n = 6) 或无生长 (n = 1) 的菌株均在推定的支链淀粉酶 I 型蛋白中携带 N 末端缺失（分别为 29 和 37 个氨基酸缺失）。从 LVM 分离的卷曲乳杆菌菌株与从 DVM 分离的卷曲乳杆菌菌株在表型上没有差异；然而，后者更有可能携带 3 片段糖基转移酶基因的发现可能表明细胞表面糖复合物的作用，这可能会影响阴道微生物群与宿主的相互作用。此外，I 型普鲁兰酶基因的变异与糖原生长相关的观察结果打消了先前关于卷曲乳杆菌不能直接利用糖原的说法。
A vaginal microbiota dominated by lactobacilli (particularly Lactobacillus crispatus) is associated with vaginal health, whereas a vaginal microbiota not dominated by lactobacilli is considered dysbiotic. Here we investigated whether L. crispatus strains isolated from the vaginal tract of women with Lactobacillus-dominated vaginal microbiota (LVM) are pheno- or genotypically distinct from L. crispatus strains isolated from vaginal samples with dysbiotic vaginal microbiota (DVM). We studied 33 L. crispatus strains (n = 16 from LVM; n = 17 from DVM). Comparison of these two groups of strains showed that, although strain differences existed, both groups degraded various carbohydrates, produced similar amounts of organic acids, inhibited Neisseria gonorrhoeae growth, and did not produce biofilms. Comparative genomics analyses of 28 strains (n = 12 LVM; n = 16 DVM) revealed a novel, 3-fragmented glycosyltransferase gene that was more prevalent among strains isolated from DVM. Most L. crispatus strains showed growth on glycogen-supplemented growth media. Strains that showed less-efficient (n = 6) or no (n = 1) growth on glycogen all carried N-terminal deletions (respectively, 29 and 37 amino acid deletions) in a putative pullulanase type I protein. L. crispatus strains isolated from LVM were not phenotypically distinct from L. crispatus strains isolated from DVM; however, the finding that the latter were more likely to carry a 3-fragmented glycosyltransferase gene may indicate a role for cell surface glycoconjugates, which may shape vaginal microbiota-host interactions. Furthermore, the observation that variation in the pullulanase type I gene is associated with growth on glycogen discourages previous claims that L. crispatus cannot directly utilize glycogen.