Lipid peroxidation and antioxidant status following thrombolytic therapy for acute myocardial infarction.
Lipid peroxidation and antioxidant status following thrombolytic therapy for acute myocardial infarction.
复制标题
急性心肌梗死溶栓治疗后的脂质过氧化和抗氧化状态。
作者：
I. S. Young;J. Purvis;J. Lightbody;A. Adgey;E. Trimble
我们研究了急性心肌梗死早期溶栓治疗成功后脂质过氧化作用增加的时间尺度，并首次报道了血浆链断裂抗氧化剂的相互变化。在症状发作后6小时内首次急性心肌梗死后招募了67例患者，并接受了70或100 mg重组组织型纤溶酶原激活剂（爱通立）两次静脉推注，间隔30分钟。分别于溶栓前、溶栓后30 min、60 min、90 min、6 h、24 h采集静脉血，观察溶栓前后血浆中血栓素的变化。在90 min时通过冠状动脉造影评估冠状动脉通畅性。丙二醛（MDA），脂质过氧化的标志物，和断链抗氧化剂α-生育酚，视黄醇和抗坏血酸通过高效液相色谱法测定。当冠状动脉通畅时，血浆MDA早期升高，（时间0 0.91 +/- 0.05 mumol.l-1），水平在90 min时达到峰值（1.02 +/- 0.06，P < 0.05），并在6 h时恢复至基线水平（0.85 +/- 0.06），伴随着α-生育酚的倒数下降（时间0 7.13 +/- 0.34 mumol.mmol-1胆固醇，90 min 6.64 +/- 0.33，P < 0.05）和视黄醇（时间0 1.99 +/- 0.10 mumol.l-1，90 min 1.81 +/- 0.08，P < 0.05）。抗坏血酸水平直到24小时才发生显著变化（时间0为29.5 +/- 4.9 μ mol.l-1，24小时为22.6 +/- 4.4，P < 0.05）。（250字处删节）
We have investigated the timescale of increased lipid peroxidation following successful early thrombolytic therapy for acute myocardial infarction and report for the first time reciprocal changes in plasma chain-breaking antioxidants. Sixty-seven patients were recruited following a first acute myocardial infarction within 6 h of the onset of symptoms and received 70 or 100 mg of recombinant tissue plasminogen activator (Actilyse) as two intravenous bolus injections 30 min apart. Serial blood samples were taken before administration of thrombolytic therapy and after 30 min, 60 min, 90 min, 6 h and 24 h. Coronary artery patency was assessed at 90 min by coronary angiography. Malondialdehyde (MDA), a marker of lipid peroxidation, and the chain-breaking antioxidants alpha-tocopherol, retinol and ascorbate were measured by high performance liquid chromatography. When the coronary artery was patent there was an early rise in plasma MDA (time 0 0.91 +/- 0.05 mumol.l-1) with levels peaking at 90 min (1.02 +/- 0.06, P < 0.05) and returning to baseline by 6 h (0.85 +/- 0.06), accompanied by reciprocal decreases in alpha-tocopherol (time 0 7.13 +/- 0.34 mumol.mmol-1 cholesterol, 90 min 6.64 +/- 0.33, P < 0.05) and retinol (time 0 1.99 +/- 0.10 mumol.l-1, 90 min 1.81 +/- 0.08, P < 0.05). Ascorbate levels did not change significantly until 24 h (time 0 29.5 +/- 4.9 mumol.l-1, 24 h 22.6 +/- 4.4, P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)