PHOTODYNAMIC INACTIVATION OF INFECTIVITY OF HUMAN-IMMUNODEFICIENCY-VIRUS AND OTHER ENVELOPED VIRUSES USING HYPERICIN AND ROSE-BENGAL - INHIBITION OF FUSION AND SYNCYTIA FORMATION
PHOTODYNAMIC INACTIVATION OF INFECTIVITY OF HUMAN-IMMUNODEFICIENCY-VIRUS AND OTHER ENVELOPED VIRUSES USING HYPERICIN AND ROSE-BENGAL - INHIBITION OF FUSION AND SYNCYTIA FORMATION
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DOI：
10.1073/pnas.90.1.158
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发表时间：
1993-01-01
影响因子：
11.1
通讯作者：
VANDEROEF, R
中科院分区：
文献类型：
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作者：
LENARD, J;RABSON, A;VANDEROEF, R
对金丝桃素的抗病毒作用机制进行了初步探讨，并与玫瑰红进行了比较。两种化合物在可见光照射下均能杀灭有包膜（但非无包膜）病毒。人类免疫缺陷病毒和水泡性口炎病毒的光动力学灭活两种染料在纳摩尔浓度。水泡性口炎、流感和仙台病毒引起的融合的光动力学灭活（溶血）由两种染料在类似条件下诱导（例如，对于水泡性口炎病毒，I50 = 20-50 nM），这表明感染性的丧失是由于融合的失活所致。合胞体的形成，激活表达人类免疫缺陷病毒gp 120在其表面和CD 4+细胞之间的细胞，被抑制的照明中存在的1 μ M金丝桃素。金丝桃素和玫瑰红因此发挥类似的杀病毒作用。两者可能通过相同的机制起作用，即通过光照产生的单线态氧使病毒融合功能失活。讨论了金丝桃素和玫瑰红的光动力抗病毒作用对潜在治疗作用的影响。
The mechanism of the antiviral activity of hypericin was characterized and compared with that of rose bengal. Both compounds inactivate enveloped (but not unenveloped) viruses upon illumination by visible light. Human immunodeficiency and vesicular stomatitis viruses were photodynamically inactivated by both dyes at nanomolar concentrations. Photodynamic inactivation of fusion (hemolysis) by vesicular stomatitis, influenza, and Sendai viruses was induced by both dyes under similar conditions (e.g., I50 = 20-50 nM for vesicular stomatitis virus), suggesting that loss of infectivity resulted from inactivation of fusion. Syncytium formation, between cells activated to express human immunodeficiency virus gp120 on their surfaces and CD4+ cells, was inhibited by illumination in the presence of 1 muM hypericin. Hypericin and rose bengal thus exert similar virucidal effects. Both presumably act by the same mechanism-namely, the inactivation of the viral fusion function by singlet oxygen produced upon illumination. The implications of this photodynamic antiviral action for the potential therapeutic usefulness of both hypericin and rose bengal are discussed.