PICOT is a molecule which binds to anamorsin
PICOT is a molecule which binds to anamorsin
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DOI：
10.1016/j.bbrc.2011.04.033
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发表时间：
2011-05-06
影响因子：
3.1
通讯作者：
Kanakura, Yuzuru
中科院分区：
文献类型：
--
作者：
Saito, Yuri;Shibayama, Hirohiko;Kanakura, Yuzuru
Anamorsin（AM）（也称为CIAPIN-1）是一种细胞死亡抵抗因子。AM缺陷型小鼠在妊娠晚期死亡; AM缺陷型胚胎与野生型（WT）胚胎相比贫血且非常小。AM被认为在造血和胚胎发生中起重要作用。为了阐明AM功能的机制，我们进行了酵母双杂交试验，以确定AM相互作用的分子，我们发现PICOT（硫氧还蛋白的PKC θ相互作用的表亲）优先结合AM。我们还发现，AM和PICOT的N端区域是它们结合所必需的，抑制两种分子的相互作用可能导致细胞生长迟缓。已知PICOT和AM的酵母同系物都是铁硫蛋白。PICOT缺陷型小鼠的表型与anamorsin缺陷型小鼠的表型非常相似;两种小鼠均具有胚胎致死性。这些数据表明，AM和PICOT可能发挥协同作用的铁硫簇蛋白在胚胎发生中的重要作用。(C)2011 Elsevier Inc. All rights reserved.
Anamorsin (AM) (also called CIAPIN-1) is a cell-death-defying factor. AM deficient mice die during late gestation; AM deficient embryos are anemic and very small compared to wild type (WT) embryos. It is thought that AM plays crucial roles in hematopoiesis and embryogenesis. To clarify the mechanisms of AM functions, we performed the yeast-two-hybrid assay to identify AM-interacting molecules; we found that PICOT (PKC theta interacting cousin of thioredoxin) preferentially bound to AM. We also showed that the N-terminal regions of both AM and PICOT were essential for their bindings and the inhibition of interaction of both molecules might lead to the cell growth retardation. Both PICOT and the yeast homolog of AM are known to be iron-sulfur proteins. The phenotype of PICOT deficient mice is very similar to that of anamorsin deficient mice; both mice are embryonic lethal. These data suggest that AM and PICOT might play cooperatively essential roles in embryogenesis as iron-sulfur cluster proteins. (C) 2011 Elsevier Inc. All rights reserved.