Inhibition mechanism of Gosha-jinki-gan on the micturition reflex in rats
Inhibition mechanism of Gosha-jinki-gan on the micturition reflex in rats
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DOI：
10.1254/jphs.fpj03098x
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发表时间：
2004-10-01
影响因子：
3.5
通讯作者：
Kamidono, S
中科院分区：
文献类型：
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作者：
Gotoh, A;Goto, K;Kamidono, S
我们研究了含有加工过的块根的日本传统药物Gosha-jinki-gan对麻醉大鼠膀胱功能的作用。在膀胱测量研究中，Gosho-jinki-gan（1.0g/kg，i.d.）增加膀胱容量以及排尿阈值压力。此外，它降低了膨胀引起的节律性膀胱收缩的频率。然而，它不影响电刺激脑桥排尿中枢引起的膀胱收缩幅度。去甲binaltorphimine（10 mg/kg，s.c.）预处理可消除Gosha-jinki-gan对膀胱运动的抑制作用，并通过同时使用抗强啡肽A抗血清（10 μ g，i.t.）而减少，育亨宾（10 μ g，i.t.），或麦角新碱（20杯，注射）。处理的块根提取物（27 mg/kg，i.d.）还抑制膀胱运动，并且该作用被去甲binaltorphimine消除。这些结果表明，Gosha-jinki-gan减弱膀胱感觉通过κ-阿片受体刺激作用的加工块茎。Gosha-jinki-gan可能是一种有用的抗尿频剂，不影响排尿时的膀胱收缩力。
We investigated the actions of Gosha-jinki-gan, a traditional Japanese medicine containing processed Aconiti tubers, on urinary bladder function in anesthetized rats. In cystometrical investigations, Gosho-jinki-gan (1.0 g/kg, i.d.) increased bladder capacity as well as micturition threshold pressure. In addition, it decreased the frequency of distension-induced rhythmic bladder contractions. However, it did not influence the amplitude of bladder contractions induced by electrical stimulation of the pontine micturition center. The inhibitory effect of Gosha-jinki-gan on bladder motility was abolished by pretreatment with nor-binaltorphimine (10 mg/kg, s.c.), and was diminished by the concomitant use of anti-dynorphin A antiserum (10 mug, i.t.), yohimbine (10 mug, i.t.), or methysergide (20 mug, i.t.). Processed Aconiti tuber extract (27 mg/kg, i.d.) also suppressed bladder motility, and the effect was abolished by nor-binaltorphimine. These results suggest that Gosha-jinki-gan attenuates bladder sensation via the kappa-opioid receptor-stimulating action of processed Aconiti tuber. Gosha-jinki-gan may be a useful anti-pollakiuria agent that does not influence bladder contractility at micturition.