Hippocampal cell alterations induced by the inhalation of vanadium pentoxide (V2O5) promote memory deterioration
Hippocampal cell alterations induced by the inhalation of vanadium pentoxide (V2O5) promote memory deterioration
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DOI：
10.1016/j.neuro.2006.04.001
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发表时间：
2006-12-01
期刊：
影响因子：
3.4
通讯作者：
Anaya-Martinez, Veronica
中科院分区：
文献类型：
--
作者：
Avila-Costa, Maria Rosa;Fortoul, Teresa I.;Anaya-Martinez, Veronica
空间记忆可能会由于衰老和神经退行性疾病而严重受损，这些疾病包括神经元损伤。钒（V）是一种广泛分布于环境中的类金属，对多种生物系统具有严重的毒性作用。关于V吸入对CNS毒性的报道有限，因此本研究的目的是确定五氧化二钒（V2 O 5）吸入（0.02 M）对记忆的影响及其与海马CA 1区细胞学的相关性。将48只CD-1雄性小鼠进行空间记忆训练，每周两次吸入IIt，每次吸入后，从1至4周对动物进行评价和处死，灌流并处理用于Golgi方法和超微结构评价。细胞学分析包括计算海马CA 1区20个锥体神经元的树突棘数量，以及超微结构特征。结果表明，V吸入产生时间依赖性的树突棘的损失，坏死样细胞死亡，和臭名昭著的海马CA 1神经堆的改变，这与空间记忆障碍。我们的数据表明，V诱导重要的细胞和功能的改变，值得特别关注的事实，因为浓度的趋势，这种元素在大气中正在增加。(c)2006年爱思唯尔公司All rights reserved.
Spatial memory may be severely impaired as a consequence of ageing and neurodegenerative diseases, conditions that include neuronal damage. Vanadium (V) is a metalloid widely distributed in the environment and exerts severe toxic effects on a wide variety of biological systems. Reports about V inhalation toxicity on the CNS are limited, thus the purpose of this study is to determine the effects of Vanadium pentoxide (V2O5) inhalation (0.02 M), on the memory and its correlation with the cytology of the hippocampus CA1. Forty eight CD-1 male mice were trained in spatial memory tasks and inhaled I It twice a week; after each inhalation animals were evaluated and sacrificed from I to 4 weeks, perfused and processed for Golgi method and for ultrastructure evaluation. The cytological analysis consisted in counting the number of dendritic spines of 20 pyramidal neurons of hippocampus CA1, as well as ultrastructural characteristics. Results show that V inhalation produces a time dependent loss of dendritic spines, necrotic-like cell death, and notorious alterations of the hippocampus CA1 neuropile, which correlate with spatial memory impairment. Our data suggest that V induces important cellular and functional alterations, fact that deserves special attention since the concentration's trend of this element in the atmosphere is increasing. (c) 2006 Elsevier Inc. All rights reserved.