Antibacterial effect of a resin incorporating a novel polymerizable quaternary ammonium salt MAE-DB against Streptococcus mutans
Antibacterial effect of a resin incorporating a novel polymerizable quaternary ammonium salt MAE-DB against Streptococcus mutans
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掺入新型可聚合季铵盐MAE-DB的树脂对变形链球菌的抗菌作用
DOI：
10.1002/jbm.b.32703
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发表时间：
2012-07-01
影响因子：
3.4
通讯作者：
Chen, Ji-Hua
中科院分区：
文献类型：
--
作者：
Huang, Li;Sun, Xiang;Chen, Ji-Hua
评价了MAE-DB树脂的抗菌性能及其作用机制。使用薄膜接触法测试对变形链球菌的抗菌作用，通过扫描电子显微镜和共聚焦激光扫描显微镜分别观察积聚和膜完整性。用定量PCR检测S.含有10% MAE-DB的树脂表面上的变异株葡萄糖基转移酶B（gtfB）基因。与对照组相比，在1天、7天、30天和180天，在含有10% MAE-DB的树脂上细菌生长受到抑制（p < 0.05）。对于10%-MAE-DB树脂，无论孵育时间如何，均未发现细菌活力的显著差异（p > 0.05）。含10% MAE-DB的树脂上附着的细菌数量明显低于对照。与对照相比，实验树脂中细胞膜受损的细菌比例增加。与对照组相比，10% MAE-DB降低了gtfB的表达（p < 0.05）。这些发现表明，MAE-DB可以以足够的浓度掺入树脂材料中，对S.通过减弱gtfB表达和损害膜完整性来抑制聚合后的变形杆菌。(c)2012 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater，2012.
The antibacterial properties of resins incorporating MAE-DB and the underlying mechanisms of action were evaluated. Antibacterial effects against Streptococcus mutans were tested using the film contact method, with accumulation and membrane integrity observed by scanning electron microscopy and confocal laser scanning microscopy, respectively. Quantitative PCR was used to determine expression of the S. mutans glucosyltransferase B (gtfB) gene on the surface of resins containing 10% MAE-DB. Bacterial growth was inhibited on resin containing 10% MAE-DB as compared with the control at 1 day, 7 days, 30 days, or 180 days (p < 0.05). For the 10%-MAE-DB resin, no significant differences in bacterial viability were found regardless of the time of incubation (p > 0.05). The number of bacteria attached to resin containing 10% MAE-DB was considerably lower than the control. The proportion of bacteria with damaged cell membranes was increased in the experimental resin over controls. Expression of gtfB was reduced by 10% MAE-DB compared with the control (p < 0.05). These findings demonstrate that MAE-DB can be incorporated into resin materials at sufficient concentrations for long-term antibacterial effects against S. mutans after polymerization by attenuating gtfB expression and impairing membrane integrity. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.