Activation of a 5-HT3 receptor releases dopamine from rat striatal slice.
Activation of a 5-HT3 receptor releases dopamine from rat striatal slice.
复制标题
5-HT3 受体的激活会从大鼠纹状体切片中释放多巴胺。
DOI：
10.1016/0014-2999(88)90528-6
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发表时间：
1988
影响因子：
5
通讯作者：
Green,JP
中科院分区：
文献类型：
--
作者：
Blandina,P;Goldfarb,J;Green,JP
5-HT 3 receptors in the periphery mediate depolarizing effects of 5-HT on neural tissue (see Richardson and Buchheit, 1988). Recently 5-HT 3 binding sites have been found in the brain (Kilpatrick et al., 1987). No certain functional correlates for these binding sites have been identified although behavioral effects of selective 5-HT 3 antagonists have been described (eg, Costall et al., 1987). We now report that activation of the 5-HT 3 receptor markedly increases the release of dopamine (DA) from rat striatal slices. Striatal slices (400/~ m thick) from eight-week old, male Sprague-Dawley rats were superfused in a thermostatic chamber (37 C, 300/~ 1 volume), at a rate of 0.5 ml/min with artificial cerebrospinal fluid (aCSF; see figure legend for composition) containing tyrosine (50/~ M), nomifensine (10/~ M) to block DA reuptake, and pargyline (10/~ M) to block monoamine oxidase, aCSF was continously gassed with 95% 02 and 5% CO 2. Sixty minutes after the start of superfusion, 1.5 ml fractions were collected into tubes containing 166# 1 of a solution of 1 N perchloric acid, 10/~ M ascorbic acid and 2.5 pg epinine (internal standard). Slices were exposed to the substances under investigation by transferring the chamber inflow tubing to reservoirs containing aCSF of appropriate composition. Samples were kept on ice during the collection period and stored at-20 C until assayed.
