Bacteriophage lambda int protein recognizes two classes of sequence in the phage att site: characterization of arm-type sites.
Bacteriophage lambda int protein recognizes two classes of sequence in the phage att site: characterization of arm-type sites.
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噬菌体 lambda int 蛋白可识别噬菌体 att 位点中的两类序列：臂型位点的表征。
DOI：
10.1073/pnas.79.24.7724
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发表时间：
1982
影响因子：
11.1
通讯作者：
Landy,A
中科院分区：
文献类型：
--
作者：
Ross,W;Landy,A
从噬菌体中纯化的蛋白结合到DNA中不属于功能附着位点（非att DNA）的特定位点以及att DNA中的特定位点。对不受核酸酶保护的非att位点的分析，确定了两种明显不同的共识识别序列，其中一种是臂型序列，在本报告中得到了描述。这两种类型的识别序列都发生在attP中；臂型共识序列的5个拷贝位于P1、P2和P臂保护区内远离交叉区。第二种类型的识别序列发生在交叉区域。用n -乙基马来酰亚胺修饰int选择性地改变其与臂型序列的相互作用。
Purified int protein from bacteriophage lambda binds to specific sites in DNA that are not part of the functional attachment sites (non-att DNA) as well as to specific sites in att DNA. Analysis of non-att sites protected from nucleases by int has permitted definition of two distinctly different consensus recognition sequences, one of which, the arm-type sequence, is characterized in this report. Both types of recognition sequence occur in attP; five copies of the arm-type consensus sequence are located distant from the crossover region in the P1, P2, and P' arm protected regions. The second type of recognition sequence occurs at the crossover region. Modification of int with N-ethylmaleimide selectively alters its interaction with arm-type sequences.