A New Analytical Method for Direct Measurement of Particulate and Dissolved B-vitamins and Their Congeners in Seawater
A New Analytical Method for Direct Measurement of Particulate and Dissolved B-vitamins and Their Congeners in Seawater
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直接测量海水中颗粒和溶解 B 族维生素及其同系物的新分析方法
DOI：
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发表时间：
2017
影响因子：
3.7
通讯作者：
S. Sañudo
中科院分区：
文献类型：
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作者：
C. Suffridge;L. Cutter;S. Sañudo
我们开发了一种新的方法，可以直接测量海水中维生素B的颗粒库和溶出库的浓度。维生素B是生命所必需的辅酶，然而许多生物不能从头合成这些化合物，必须从环境中清除它们。已有研究表明，B族维生素可以控制海洋微生物群落的结构和活动；然而，由于缺乏分析方案，海洋微生物的实际B族维生素需要量/定额没有得到很好的研究。这种方法将使研究维生素B的细胞配额及其环境循环成为可能。用这种方法，我们还可以同时测定微粒和溶解水池中维生素B1和B12以及维生素B2、B6、B7和氨基酸蛋氨酸的生化同系物，经过化学提取(微粒)或树脂预浓缩(溶解)后，使用液-质联用技术。从大西洋的微生物群落中同时测定了微粒和溶解的B族维生素浓度。大西洋中的微粒B-维生素浓度从0.01 PM(氰基-B12)到46.4 PM(硫胺素单磷酸盐，一种B1同系物)，而溶解的B-维生素浓度从0.07 PM(腺苷-B12)到679.4 PM(B7)。这项技术在不同海洋系统中的应用有可能对海洋微生物群落中发生的以前未知的生化过程以及由此产生的B族维生素在调节全球生物地球化学循环中所起的作用提供新的线索。
We have developed a new method to directly measure the concentration of the particulate and dissolved pools of B-vitamins in seawater. B-vitamins are coenzymes required for life, yet many organisms cannot synthesize these compounds de novo and must scavenge them from the environment. It has been shown that B-vitamins can control the marine microbial community’s structure and activity; however, the actual B-vitamin requirements/quotas for marine microbes are not well studied due to the lack of analytical protocols. This method will enable the study of B-vitamin cellular quotas as well as their environmental cycling. With this method, we can also simultaneously determine the biochemical congeners of vitamins B1 and B12, as well as vitamins B2, B6, B7, and the amino acid methionine in both the particulate and dissolved pool, using liquid chromatography-mass spectrometry after a chemical extraction (particulate) or resin preconcentration (dissolved). Particulate and dissolved B-vitamin concentrations were simultaneously determined from a microbial community in the Atlantic Ocean. Particulate B-vitamin concentrations in the Atlantic ranged from 0.01 pM (cyano-B12) to 46.4 pM (thiamin monophosphate, a B1 congener) while dissolved B-vitamin concentrations ranged from 0.07 pM (adenosyl-B12) to 679.4 pM (B7). The application of this technique in different marine systems has the potential to shed new light on previously unknown biochemical processes occurring within the oceanic microbial community, and the resulting roles B-vitamins play in regulating global biogeochemical cycles.