Analysis of Helicobacter pylori Genotype in Stool Specimens of Asymptomatic People
Analysis of Helicobacter pylori Genotype in Stool Specimens of Asymptomatic People
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DOI：
10.1309/lmz2wwcd2a9mftnw
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发表时间：
2009-07-01
期刊：
影响因子：
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通讯作者：
Yamamoto, Yoshimasa
中科院分区：
文献类型：
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作者：
Sasaki, Tadahiro;Hirai, Itaru;Yamamoto, Yoshimasa
背景幽门螺杆菌毒力因子基因分型在人类健康护理中具有重要意义。然而，H. pylori感染无症状的人没有得到很好的研究，由于在收集胃标本的困难。方法：我们分析了164无症状的参与者在厄瓜多尔和巴拿马利用粪便标本。采用免疫学方法检测H. pylori。H.结果：在厄瓜多尔和巴拿马分别有72.2%和54.1%的受试者存在幽门螺杆菌抗原。在H. pylori DNA阳性样本中，厄瓜多尔和巴拿马的cagA检出率分别为45.9%和20.0%。结论：本方法对我国汉族人群的cagA基因检测有一定的参考价值。pylori感染情况及H. pylori感染和/或H.幽门螺杆菌可能与胃癌的风险有关。
Background. Genotyping of Helicobacter pylori virulence factor is important in human health care. However, the genotyping of H. pylori infecting asymptomatic people is not well studied due to the difficulty in collecting gastric specimens.Methods: We analyzed 164 asymptomatic participants in Ecuador and Panama by utilizing stool specimens. H, pylori was detected using immunological methods. H. pylori virulence factor cagA was genotyped by PCR.Results: Helicobacter pylori antigen was found in 72.2% of participants from Ecuador and 54.1% from Panama. Among H. pylori DNA-positive samples, cagA was detected in 45.9% from Ecuador and 20.0% from Panama. Over 80% of the detected cagA from both countries was East-Asian genotype.Conclusions: The results suggest that our method is valuable for assessing the H. pylori infection status of asymptomatic people and that the prevalence of H. pylori infection and/or the occurrence of cagA in H. pylori may be related to the risk of stomach cancer.