IONIC TRIGGERS IN THE FERTILIZATION OF SEA URCHIN EGGS
IONIC TRIGGERS IN THE FERTILIZATION OF SEA URCHIN EGGS
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海胆卵受精过程中的离子触发因素
DOI：
10.1111/j.1749-6632.1980.tb15970.x
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发表时间：
1980
影响因子：
5.2
通讯作者：
D. Epel
中科院分区：
文献类型：
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作者：
D. Epel
我们已经考虑了钙的短暂上升是如何发生的，以及它是如何导致卵子的激活的。我们对“人造精子”的研究表明，即使精子局部释放钙，也不足以在卵子周围完全繁殖。需要别的东西，而这个东西可以通过把鸡蛋放在高于正常浓度的钾离子中来模拟。一种可能性是钾效应是由诱导膜去极化引起的，类似于精子-卵子结合后通常发生的去极化。如果是正确的，那么膜去极化可能不仅在阻断多精蛋白10中起作用，而且还可能参与“放松”或克服细胞正常的钙隔离能力。一个可能的机制是电压依赖的Ca++诱导的Ca++释放。
We have considered how a transient rise in calcium can occur and how it can result in the activation of the egg. Our evidence with the “artificial sperm”—the ionophore‐coated glass rod—suggests that even if sperm release calcium locally, this may be insufficient to completely propagate around the egg. Something else is needed and this something else can be mimicked by placing the eggs in higher than normal concentrations of potassium ion. A possibility is that the potassium effect results from inducing a membrane depolarization similar to the one that normally follows sperm‐egg binding. If correct, then the membrane depolarization might not only function in the block to polyspermy10 but could also be involved in “relaxing” or overcoming the normal calcium‐sequestering ability of the cell. A possible mechanism is a voltage‐dependent Ca++ ‐induced Ca++ release.