Inositol phosphate metabolomics: merging genetic perturbation with modernized radiolabeling methods.
Inositol phosphate metabolomics: merging genetic perturbation with modernized radiolabeling methods.
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磷酸肌醇代谢组学：将遗传扰动与现代化放射性标记方法相结合。
DOI：
10.1016/j.ymeth.2006.05.012
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
York,JohnD
中科院分区：
文献类型：
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作者：
Stevenson-Paulik,Jill;Chiou,Shean-Tai;Frederick,JoshP;delaCruz,June;Seeds,AndrewM;Otto,JamesC;York,JohnD
最近的发现提供了肌醇磷酸（IP）信号和基本细胞过程之间的联系，引起了许多令人兴奋的新的假设IP功能，并强调了解IP合成是如何调节的重要性。IP代谢研究的核心是开发有效，快速和可重复的方法，用于从简单的细胞培养到更复杂的组织和整个生物体系统中的IP定量分析。此外，在许多情况下，需要抑制和/或遗传改变IP激酶和磷酸酶活性，以使低丰度肌醇信号传导信使可视化。在这里，我们描述了更新的方法，快速分析IP代谢在正常和遗传操作的酿酒酵母，拟南芥，果蝇，小家鼠，和智人。
Recent discoveries that provide a link between inositol phosphate (IP) signaling and fundamental cellular processes evoke many exciting new hypotheses about IP function, and underscore the importance of understanding how IP synthesis is regulated. Central to studies of IP metabolism is the essential development of efficient, fast, and reproducible methods for quantitative analysis of IPs in systems ranging from simple cell cultures to more complex tissues and whole organisms. Additionally, in many cases there is a need to pharmacologically and/or genetically alter IP kinase and phosphatase activities in order to visualize low abundance inositol signaling messengers. Here, we describe updated methods for rapid analysis of IP metabolism in normal and genetically manipulated Saccharomyces cerevisiae, Arabidopsis thaliana, Drosophila melanogaster, Mus musculus, and Homo sapiens.