Depletion of intracellular calcium stores by calcium ionophore A23187 induces the genes for glucose-regulated proteins in hamster fibroblasts.
Depletion of intracellular calcium stores by calcium ionophore A23187 induces the genes for glucose-regulated proteins in hamster fibroblasts.
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DOI：
10.1016/s0021-9258(18)45277-5
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发表时间：
1987-09
期刊：
影响因子：
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通讯作者：
I. Drummond;A. Lee;E. Resendez;R. Steinhardt
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文献类型：
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作者：
I. Drummond;A. Lee;E. Resendez;R. Steinhardt
动物细胞对钙离子载体（A23187）处理的反应是通过协调诱导一组基因编码与葡萄糖调节蛋白（GRPs）相同的蛋白质。通过荧光指示剂fura-2监测细胞内游离钙，同时使用细胞内和细胞外钙缓冲液，我们证明A23187可以诱导GRP94和GRP78基因，而不增加细胞质钙（[Ca2+]i）。葡萄糖饥饿期间GRP mRNA的诱导也独立于[Ca2+]i。相反，A23187的基因诱导与细胞内钙储存的消耗密切相关。我们得出结论，A23187对螯合钙离子的扰动可以作为基因表达的刺激。
Animal cells respond to calcium ionophore (A23187) treatment with the coordinate induction of a set of genes encoding proteins identical to the glucose-regulated proteins (GRPs). By monitoring the intracellular free calcium with the fluorescent indicator fura-2 while employing both intracellular and extracellular calcium buffers, we demonstrated that A23187 can induce the GRP94 and GRP78 genes without an increase in cytoplasmic calcium ([Ca2+]i). Induction of GRP mRNA during glucose starvation was also independent of [Ca2+]i. Instead, gene induction by A23187 was closely correlated with the depletion of intracellular calcium stores. We conclude that perturbations of sequestered calcium ions by A23187 can serve as a stimulus for gene expression.