RECOVERY IN TRACHEAL ORGAN CULTURES OF NOVEL VIRUSES FROM PATIENTS WITH RESPIRATORY DISEASE
RECOVERY IN TRACHEAL ORGAN CULTURES OF NOVEL VIRUSES FROM PATIENTS WITH RESPIRATORY DISEASE
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DOI：
10.1073/pnas.57.4.933
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发表时间：
1967-01-01
影响因子：
11.1
通讯作者：
CHANOCK, RM
中科院分区：
文献类型：
--
作者：
MCINTOSH, K;DEES, JH;CHANOCK, RM
在成人急性上呼吸道疾病中，在人胚胎气管器官培养物中检查了通过标准组织培养技术未能产生病毒的鼻咽冲洗液。从 23 个样本中回收了 8 种试剂，其中 2 种似乎对乙醚稳定，只能通过其在器官培养中的纤毛固定作用来检测。其余 6 个是在通过电子显微镜检查器官培养收获物时检测到的。这些病毒表现出一种不寻常的形态，与禽传染性支气管炎病毒 (IBV) 和从人类身上发现的其他两种对乙醚不稳定的病毒非常相似：229E 株和 B814 株（一种通过器官培养传播的病毒）。对 6 种“IBV 样”病毒中的 5 种进行了检查并用乙醚灭活。该组以 IBV 为形态学原型，似乎与粘病毒不同。
In acute upper respiratory tract disease in adults, naso-pharyngeal washings which failed to yield viruses by standard tissue culture techniques were examined in human embryonic tracheal organ cultures. From 23 specimens, 8 agents were recovered, 2 of which appeared to be ether-stable and were detectable only by their ciliary immobilizing effect in organ culture. The remaining 6 were detected when organ culture harvests were examined by electron microscopy. These viruses exhibited an unusual morphology closely resembling that of avian infectious bronchitis virus (IBV) and 2 other ether-labile agents recovered from man: strain 229E, and strain B814, an organ-culture-propagated virus. Five of the 6 "IBV-like" viruses were examined and were inactivated by ether. This group, for which IBV is the morphologic prototype, appears to be distinct from the myxoviruses.