Mutation of sarA in Staphylococcus aureus limits biofilm formation
Mutation of sarA in Staphylococcus aureus limits biofilm formation
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DOI：
10.1128/iai.71.7.4206-4211.2003
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发表时间：
2003-07-01
影响因子：
3.1
通讯作者：
Smeltzer, MS
中科院分区：
文献类型：
--
作者：
Beenken, KE;Blevins, JS;Smeltzer, MS
sarA 突变导致我们测试的八种金黄色葡萄球菌菌株中的六种（UAMS-1、UAMS-601、SA113、SC-01、S6C 和 DB）形成生物膜的能力降低。例外的是 Newman，它在所有条件下都形成了较差的生物膜，而 RN6390，它仅在 agr 突变后始终形成生物膜。其他菌株中agr的突变对生物膜的形成几乎没有影响。在除 Newman 之外的每个菌株中，包括 RN6390，sarA 和 agr 的同时突变导致与 sarA 突变体观察到的表型类似。使用 sarA 克隆的互补研究证实，生物膜形成的缺陷是由于 sarA 突变造成的。
Mutation of sarA resulted in a reduced capacity to form a biofilm in six of the eight Staphylococcus aureus strains we tested (UAMS-1, UAMS-601, SA113, SC-01, S6C, and DB). The exceptions were Newman, which formed a poor biofilm under all conditions, and RN6390, which consistently formed a biofilm only after mutation of agr. Mutation of agr in other strains had little impact on biofilm formation. In every strain other than Newman, including RN6390, simultaneous mutation of sarA and agr resulted in a phenotype like that observed with the sarA mutants. Complementation studies using a sarA clone confirmed that the defect in biofilm formation was due to the sarA mutation.