MUTATIONAL ANALYSIS OF THE HELPER COMPONENT-PROTEINASE GENE OF A POTYVIRUS - EFFECTS OF AMINO-ACID SUBSTITUTIONS, DELETIONS, AND GENE REPLACEMENT ON VIRULENCE AND APHID TRANSMISSIBILITY
MUTATIONAL ANALYSIS OF THE HELPER COMPONENT-PROTEINASE GENE OF A POTYVIRUS - EFFECTS OF AMINO-ACID SUBSTITUTIONS, DELETIONS, AND GENE REPLACEMENT ON VIRULENCE AND APHID TRANSMISSIBILITY
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DOI：
10.1073/pnas.90.24.11919
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发表时间：
1993-12-15
影响因子：
11.1
通讯作者：
PIRONE, TP
中科院分区：
文献类型：
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作者：
ATREYA, CD;PIRONE, TP
我们之前提供的证据表明，烟草脉斑病毒（TVMV）辅助成分蛋白酶（HC-Pro）N末端部分的氨基酸取代，特别是在LyS-307处，不仅影响HC-Pro的蚜虫传播活性，而且还对TVMV毒力有显着影响。在本研究中，在307位取代了电荷性质不同的氨基酸。需要高度碱性的残基来保持辅助组分的活性和毒力。HC-Pro基因5'端的缺失和插入突变表明，该RNA结构域可能是TVMV感染性的必需元件。替换TVMV HC-Pro基因，从另一个马铃薯Y病毒，小西葫芦黄色花叶病毒，保持感染性和蚜虫传播的嵌合病毒，虽然症状减弱。我们的研究结果表明，除了其在蚜虫传播的重要性，HC-Pro基因可能是普遍的重要性，在调节马铃薯Y病毒的毒力，可能通过这些序列与宿主的相互作用。
We have previously provided evidence that amino acid substitutions within the N-terminal portion of the helper component-proteinase (HC-Pro) from tobacco vein mottling virus (TVMV), in particular at Lys-307, not only affect the aphid transmission activity of HC-Pro but also have a significant effect on TVMV virulence. In the present study amino acids which differ in their charge properties were substituted at position 307. A highly basic residue was required to retain helper component activity and virulence. Deletion and insertion mutagenesis in the 5' terminus of the HC-Pro gene suggested that this RNA domain may be an essential element for TVMV infectivity. Replacement of the TVMV HC-Pro gene with that from another potyvirus, zucchini yellow mosaic virus, maintained infectivity and aphid transmissibility of the chimeric virus, although symptoms were attenuated. Our results suggest that, in addition to its importance in aphid transmission, the HC-Pro gene may be of general importance in regulating virulence of potyviruses, possibly by interaction of these sequences with the host.