Diagnosis of Gestational, Congenital, and Placental Malaria in Colombia: Comparison of the Efficacy of Microscopy, Nested Polymerase Chain Reaction, and Histopathology
Diagnosis of Gestational, Congenital, and Placental Malaria in Colombia: Comparison of the Efficacy of Microscopy, Nested Polymerase Chain Reaction, and Histopathology
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DOI：
10.4269/ajtmh.2011.10-0507
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发表时间：
2011-06-01
影响因子：
3.3
通讯作者：
Maestre, Amanda
中科院分区：
文献类型：
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作者：
Campos, Ivon M.;Uribe, Mary L.;Maestre, Amanda
在哥伦比亚尚未对不同方法诊断母体外周血、胎盘和脐带血中疟原虫的技术能力进行评估，在其他疟疾流行地区也很少进行探讨。我们设计了一项研究来比较光学显微镜（LM）、巢式聚合酶链反应（nPCR）和组织病理学（HP）的技术和操作经济性能。与nPCR相比，LM在母体血液中的敏感性为41%，特异性为100%，在胎盘血液中的敏感性为35%，特异性为100%。在胎盘组织中，LM的敏感性为33%，特异性为95%；和nPCR分别为47%和77%；与惠普相比。光学显微镜具有最佳的操作经济性。我们得出结论，nPCR和HP比LM表现更好，但这两种技术的现场实施仍然是一个问题。因此，LM被推荐作为诊断妊娠期疟疾和胎盘血液感染的金标准。
The technical capability of different methods to diagnose Plasmodium in maternal peripheral blood, placenta, and umbilical cord blood has not been assessed in Colombia and seldom explored in other malaria-endemic regions. We designed a study to compare the technical and the operational-economical performances of light microscopy (LM), nested polymerase chain reaction (nPCR), and histopathology (HP). In maternal blood, LM had 41% sensitivity and 100% specificity and in placental blood, 35% and 100%, respectively, compared with nPCR. In placental tissue, LM had 33% sensitivity and 95% specificity; and nPCR 47% and 77%, respectively; compared with HP. Light microscopy had the best operational-economical qualification. We concluded that nPCR and HP performed better compared with LM, but field implementation of these two techniques remains a problem. Therefore, LM is recommended as the gold standard for diagnosis of gestational malaria and placental blood infection in the field.