VERSICAN, A HYALURONATE-BINDING PROTEOGLYCAN OF EMBRYONAL PRECARTILAGINOUS MESENCHYMA, IS MAINLY EXPRESSED POSTNATALLY IN RAT-BRAIN
VERSICAN, A HYALURONATE-BINDING PROTEOGLYCAN OF EMBRYONAL PRECARTILAGINOUS MESENCHYMA, IS MAINLY EXPRESSED POSTNATALLY IN RAT-BRAIN
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DOI：
10.1002/jnr.490340110
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发表时间：
1993-01-01
影响因子：
4.2
通讯作者：
RAHEMTULLA, F
中科院分区：
文献类型：
--
作者：
BIGNAMI, A;PERIDES, G;RAHEMTULLA, F
在大鼠产前和产后发育中研究了多功能蛋白聚糖（一种大型透明质酸结合成纤维细胞蛋白多糖）的定位。在成年大鼠白质和小脑中，多功能蛋白聚糖的分布与之前报道的脑特异性神经胶质透明质酸结合蛋白（GHAP）的分布相同。在灰质中也发现了多功能蛋白聚糖，它在大神经元周围形成了特征性的涂层。它还存在于外周组织中，即肾髓质、肌腱连接处以及神经内膜和肌内膜鞘。在大鼠胚胎中，最显着的发现是软骨前间质中存在大量多功能蛋白聚糖免疫反应物质。在胚胎中枢神经系统中，多功能蛋白聚糖主要局限于大脑半球表面的边缘区。 Versacan 表达主要发生在出生后的脑和脊髓中。在脊髓白质中，多功能蛋白聚糖免疫反应性已在 3 日龄大鼠中出现，并且先于 GHAP 出现，GHAP 在髓鞘形成开始后第 13 天首次检测到。灰质中Versacan的表达明显延迟。第 21 天，在大脑皮层首次观察到特征性的神经周围皮层。结论是，除透明质酸外，脑细胞外基质（ECM）主要在出生后产生，并且脑细胞（最有可能是星形胶质细胞）产生的ECM蛋白与软骨前间质产生的相似。
The localization of versican, a large hyaluronate-binding fibroblast proteoglycan, was studied in rat prenatal and postnatal development. In adult rat white matter and cerebellum, the distribution of versican was identical to that previously reported for brain-specific glial hyaluronate-binding protein (GHAP). Versican was also found in gray matter where it formed characteristic coats around large neurons. It was also found in peripheral tissues, namely, kidney medulla, myotendinous junctions, and endoneurial and endomysial sheaths. In rat embryo the most notable finding was the presence of large amounts of versican immunoreactive material in precartilaginous mesenchyma. In embryonal CNS, versican was mainly confined to the marginal zone on the surface of the cerebral hemispheres. Versican expression mainly occurred postnatally in brain and spinal cord. In spinal cord white matter, versican immunoreactivity was already present in 3-day-old rats and preceded the appearance of GHAP, which was first detected on day 13 after the onset of myelination. Versican expression was markedly delayed in gray matter. The characteristic perineuronal coats were first observed on day 21 in the cerebral cortex. It is concluded that, with the exception of hyaluronate, brain extracellular matrix (ECM) is mainly produced postnatally and that the ECM protein produced by brain cells, most likely astrocytes, is similar to that produced by precartilaginous mesenchyma.