Effects of gold nanostructures on differentiation of mesenchymal stem cells
Effects of gold nanostructures on differentiation of mesenchymal stem cells
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金纳米结构对间充质干细胞分化的影响
DOI：
10.1016/j.colsurfb.2019.110494
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发表时间：
2019-12-01
影响因子：
5.8
通讯作者：
Deng，Jun
中科院分区：
文献类型：
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作者：
Yuan，Long;Qi，Xiaowei;Deng，Jun
纳米粒子是纳米晶体，具有复杂的面和有缺陷的结构，不会采用理想的形状。纳米粒子的表面组成、大小、硬度等物理化学参数都可以调节间充质干细胞的分化，但多个边缘和角区的形状对其分化的影响尚未被研究。在本研究中，我们研究了两种经11-巯基十一酸修饰的金纳米结构，即金纳米立方体(MUA-AuNC)和纳米八面体(MUA-AuNO)对大鼠骨髓间充质干细胞(BMSCs)活性和分化的影响。细胞毒性和增殖分析显示，细胞具有良好的生物相容性，浓度为100μg·mL−-1与未经处理的对照组相比无显著差异。碱性磷酸酶活性和茜素红S染色显示，低浓度(5μg·m L−1)和高浓度(2 5μg·m L−1)的金纳米结构处理后，骨髓间充质干细胞向成骨细胞分化的能力较弱。相反，油红O染色显示，两种纳米结构都促进了成脂分化，并在基因和蛋白水平上调了过氧化体增殖物激活受体γ(PPARγ)和脂肪酸结合蛋白4(FABP4)的表达。MUA-AuNO对成骨细胞分化的影响具有结构和剂量依赖性；MUA-AuNO更有效地促进成脂分化和减弱成骨分化，可能是由于产生了更高水平的ROS。这些发现为将这些纳米粒子用于基于MSC的成像和治疗奠定了基础。
Nanoparticles are nanocrystals with complex facets and defective structures that do not adopt an idealised shape. Various physicochemical parameters of nanoparticles, such as surface composition, size, and stiffness, can regulate differentiation in mesenchymal stem cells (MSCs), but the influence of shapes with many edges and corner regions has not been investigated. Herein, we investigated the effects of two gold nanostructures modified with 11-mercaptoundecanoic acid, namely gold nanocubes (MUA-AuNCs) and nanooctahedras (MUA-AuNOs), on viability and differentiation in rat bone marrow MSCs (bMSCs). Analysis of cytotoxicity and proliferation demonstrated good biocompatibility, with concentrations <100μg·mL−1not significantly different from untreated controls. Alkaline phosphatase activity and Alizarin Red S staining revealed weaker potential for bMSCs to differentiate into osteoblasts following treatment with both low (5μg·mL−1) and high (25μg·mL−1) concentrations of the gold nanostructures. By contrast, Oil Red O staining showed that both nanostructures enhanced adipogenic differentiation, and upregulated peroxisome proliferator-activated receptor gamma (PPARγ) and fatty acid binding protein-4 (Fabp4) expression at both mRNA and protein levels. The effects on differentiation were both structure- and dose-dependent; MUA-AuNOs were more effective for enhancing adipogenic differentiation and weakening osteogenic differentiation, possibly due to generating higher levels of reactive oxygen species (ROS). These findings lay the foundation for using these nanoparticles asex vivolabels in MSC-based imaging and therapy.