Streptococcus Mutans Adhesion to Titanium After Brushing with Fluoride and Fluoride-Free Toothpaste Simulating 10 Years of Use
Streptococcus Mutans Adhesion to Titanium After Brushing with Fluoride and Fluoride-Free Toothpaste Simulating 10 Years of Use
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DOI：
10.11607/jomi.2452
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发表时间：
2013-03-01
影响因子：
2
通讯作者：
Adabo, Gelson L.
中科院分区：
文献类型：
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作者：
Fais, Laiza M. G.;Carmello, Juliana C.;Adabo, Gelson L.
目的：使用模拟 10 年刷牙的实验范例来评估氟化物对变形链球菌与钛的粘附的影响。材料和方法：对商业纯钛 (cpTi) 和钛合金 (Ti-6Al-4V) 圆盘（直径 6 mm，厚 4 mm）进行镜面抛光，并随机分配到以下六组之一 (n = 6)：去离子水中浸泡 (I) 或刷牙 (B)（IW 组 [对照组] 和 BW）、无氟牙膏（IT 组和 BT 组）或氟化牙膏（组） IFT 和 BFT）。将浸泡过的样品静态浸入溶液中，无需刷洗。对于刷过的样本，使用带有软毛牙刷的线性刷牙机。实验总共持续了244小时。处理前后，在原子力显微镜下对样品进行分析，以确定平均粗糙度（Ra）和轮廓最大峰谷高度平均值（Rtm）。在含有脑心浸液肉汤的孔板中，圆盘被标准变形链球菌菌株污染。使用扫描电子显微镜根据粘附的活细胞的集落形成单位（CFU/mL）的数量来分析粘附。通过单向方差分析来分析各组之间 CFU/mL 的差异。结果：浸没不会影响任何一个表面。 Ra 和 Rtm 表明，BW、BT 和 BFT 会引起 cpTi 表面的变化，而只有 BT 和 BTF 会引起 Ti-6Al-4V 表面的变化。 cpTi 或 Ti-6Al-4V 组之间的 CFU/mL 没有观察到显着差异。所有表面的变形链球菌粘附力相似。结论：用含氟牙膏模拟刷牙10年引起的钛变化并没有增加变形链球菌的粘附力。
Purpose: To assess the influence of fluoride on the adhesion of Streptococcus mutans to titanium using an experimental paradigm simulating 10 years of brushing. Materials and Methods: Commercially pure titanium (cpTi) and titanium alloy (Ti-6Al-4V) disks (6 mm in diameter and 4 mm thick) were mirror-polished and randomly assigned to one of the following six groups (n = 6): immersion (I) or brushing (B) in deionized water (groups IW [control] and BW), fluoride-free toothpaste (groups IT and BT), or fluoridated toothpaste (groups IFT and BFT). Specimens subjected to immersion were statically submerged into the solutions without brushing. For the brushed specimens, a linear brushing machine with a soft-bristled toothbrush was used. The experiments lasted a total of 244 hours. Before and after treatment, the specimens were analyzed under an atomic force microscope to determine the mean roughness (Ra) and the mean of the maximum peak-to-valley heights of the profile (Rtm). The disks were contaminated with standard strains of S mutans in well plates with brain-heart infusion broth. Adhesion was analyzed based on the numbers of colony-forming units (CFU/mL) of adhered viable cells using scanning electronic microscopy. Differences in CFU/mL between the groups were analyzed by one-way analysis of variance. Results: Immersion did not affect either surface. As suggested by Ra and Rtm, BW, BT, and BFT induced changes on the surface of cpTi, whereas only BT and BTF induced changes on the surface of Ti-6Al-4V. No significant differences were observed regarding CFU/mL among the cpTi or Ti-6Al-4V groups. S mutans adhesion was similar for all surfaces. Conclusions: The changes in titanium induced by 10 years of simulated brushing with fluoride toothpaste did not increase the adhesion of S mutans.