Fabrication of column chip made of PMMA for μFIA
Fabrication of column chip made of PMMA for μFIA
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DOI：
10.1016/j.talanta.2011.04.053
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发表时间：
2011-07-15
期刊：
影响因子：
6.1
通讯作者：
Suzuki, Koji
中科院分区：
文献类型：
--
作者：
Ito, Takeshi;Kaneko, Satoru;Suzuki, Koji
我们提出了一种低成本的聚甲基丙烯酸甲酯（PMMA）柱芯片的制造工艺。采用干膜光刻技术和光刻工艺在芯片上制作了一个由四个圆柱组成的三维微通道结构。对母模进行电铸，得到镍模，然后通过热压将图案转移到PMMA上。该柱具有坝结构，以保持体积为640 nL的酶固定化微珠。将该柱芯片应用于微流动注射分析系统。为了演示，我们使用两个串联的色谱柱测量乳糖。其中一个柱位于上游，填充固定化β-半乳糖苷酶的壳聚糖微球，另一个柱位于下游，填充固定化葡萄糖氧化酶的壳聚糖微球。乳糖检测在样品注射后不到90 s完成。生物传感系统也表现出高性能的乳糖检测在宽范围内的1 μ M至1 mM。这些结果表明，柱芯片和我们的微流控生物传感系统有潜力，以协助minuaturization与小样品体积和短的测定时间的顺序分析。(C)2011 Elsevier B. V.保留所有权利。
We proposed a low cost fabrication procedure of a poly(methylmethacrylate) (PMMA) column chip. 3D microchannel structure consisting of four columns in a chip for a mother die was fabricated using dry film photoresist and photolithography technique. Electroforming was applied to the mother die in order to obtain a Ni mold, then, the pattern was transferred to PMMA by hot press. The column had a dam structure to keep enzyme-immobilized microbeads with volume of 640 nL. The column chip was applied for a micro flow injection analysis (mu FIA) system. For a demonstration, we measured lactose using two columns in series. One column was set on upper stream and filled with chitosan microbeads immobilized with beta-galactosidase, the other was on downstream and filled with the beads immobilized with glucose oxidase. The lactose detection was accomplished less than 90s after the sample injection. The biosensing system also showed a high performance for lactose detection in wide range of 1 mu M to 1 mM. These results show that the column chip and our microfluidic biosensing system have the potential to assist minuaturization with small sample volume and short determination time for a sequential analysis. (C) 2011 Elsevier B.V. All rights reserved.