Bcl-x is not required for maintenance of follicles and corpus luteum in the postnatal mouse ovary.
Bcl-x is not required for maintenance of follicles and corpus luteum in the postnatal mouse ovary.
复制标题
Bcl-x 不需要维持产后小鼠卵巢中的卵泡和黄体。
DOI：
10.1095/biolreprod66.2.438
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发表时间：
2002
影响因子：
3.6
通讯作者：
Hennighausen,Lothar
中科院分区：
文献类型：
--
作者：
Riedlinger,Gregory;Okagaki,Ryugo;Wagner,Kay-Uwe;Rucker3rd,EdmundB;Oka,Takami;Miyoshi,Keiko;Flaws,JodiA;Hennighausen,Lothar
已经提出Bcl-x是许多细胞类型中的关键存活因子，并且bcl-x基因通过细胞因子信号被转录因子Stat 5激活。为了支持这一点，已经证明小鼠原始生殖细胞在胚胎发生期间的存活取决于Bcl-x的存在。我们现在已经研究了在小鼠中，Bcl-x是否是卵泡和黄体细胞出生后维持所必需的，以及Stat 5是否激活bcl-x基因。使用Cre-loxP重组在小鼠内的这些细胞中缺失bcl-x基因。bcl-x基因的缺失不影响原始卵泡、初级卵泡和有腔卵泡的数量。此外，卵巢中bcl-x基因的表达不依赖于Stat 5及其激活激素催乳素。为了确定是否催乳素受体（PrlR），Stat 5，和Bcl-x所需的建立和维护的黄体，我们诱导假孕在各自的基因缺失小鼠。而黄体细胞发生凋亡的PrlR的情况下，没有观察到的Stat 5或Bcl-x的情况下，没有变化。
It has been proposed that Bcl-x is a key survival factor in many cell types, and that thebcl-xgene is activated by the transcription factor Stat5 through cytokine signals. In support of this, it has been demonstrated that the survival of mouse primordial germ cells during embryogenesis depends on the presence of Bcl-x. We have now investigated whether, in the mouse, Bcl-x is required for the postnatal maintenance of follicles and luteal cells, and whether Stat5 activates thebcl-xgene. Thebcl-xgene was deleted in these cells within the mouse using Cre-loxP recombination. Loss of thebcl-xgene did not affect the numbers of primordial, primary, and antral follicles. Furthermore, expression of thebcl-xgene in the ovary was independent of Stat5 and its activating hormone, prolactin. To determine whether the prolactin receptor (PrlR), Stat5, and Bcl-x were required for establishment and maintenance of the corpus luteum, we induced pseudopregnancies in the respective gene-deletion mice. Whereas luteal cells underwent apoptosis in the absence of the PrlR, no changes were observed in the absence of Stat5 or Bcl-x.