Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (BARS-CoV-2) Is Comparable in Clinical Samples Preserved in Saline or Viral Transport Medium
Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (BARS-CoV-2) Is Comparable in Clinical Samples Preserved in Saline or Viral Transport Medium
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DOI：
10.1016/j.jmoldx.2020.04.209
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发表时间：
2020-07-01
影响因子：
4.1
通讯作者：
Blaser, Martin J.
中科院分区：
文献类型：
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作者：
Radbel, Jared;Jagpal, Sugeet;Blaser, Martin J.
随着2019冠状病毒病（COVID-19）大流行席卷全球，病毒转运培养基（VTM）的可用性受到严重限制，导致诊断延迟和诊断检测配给。鉴于严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）病毒RNA已被证明具有稳定性，我们假设磷酸盐缓冲液（PBS）可能是一种可行的转运介质，作为VTM的替代品，用于临床实时定量PCR（qPCR）检测。在VTM或PBS中运输的临床气管内分泌物样本中评估了SARS-CoV-2 qPCR的个体内可靠性和个体间可靠性，以评价当样本在室温下储存在这些培养基中长达18小时时，三种病毒靶标（N基因、ORF 1ab和S基因）的qPCR信号的稳定性。我们报告说，使用PBS作为运输介质允许高的个体内和个体间的可靠性，保持病毒的稳定性，并通过18小时的存储在检测三个SARS-CoV-2基因与VTM进行比较。本研究确定PBS作为临床上有用的介质，可以很容易地部署用于运输和短期保存含有SARS-CoV-2的标本。使用PBS作为转运介质有可能提高SARS-CoV-2的检测能力，有助于更广泛地筛查和早期诊断COVI D-19。
As the coronavirus disease 2019 (COVID-19) pandemic sweeps across the world, the availability of viral transport medium (VTM) has become severely limited, contributing to delays in diagnosis and rationing of diagnostic testing. Given that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA has demonstrated stability, we posited that phosphate-buffered saline (PBS) may be a viable transport medium, as an alternative to VTM, for clinical real-time quantitative PCR (qPCR) testing. The intra-individual reliability and interindividual reliability of SARS-CoV-2 qPCR were assessed in clinical endotracheal secretion samples transported in VTM or PBS to evaluate the stability of the qPCR signal for three viral targets (N gene, ORF1ab, and S gene) when samples were stored in these media at room temperature for up to 18 hours. We report that the use of PBS as a transport medium allows high intra-individual and interindividual reliability, maintains viral stability, and compares with VTM in the detection of the three SARS-CoV-2 genes through 18 hours of storage. This study establishes PBS as a clinically useful medium that can be readily deployed for transporting and short-term preservation of specimens containing SARS-CoV-2. Use of PBS as a transport medium has the potential to increase testing capacity for SARS-CoV-2, aiding more widespread screening and early diagnosis of COVI D-19.