Phenotypic conversion of drug-resistant bacteria to drug sensitivity
Phenotypic conversion of drug-resistant bacteria to drug sensitivity
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DOI：
10.1073/pnas.94.16.8468
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发表时间：
1997-08-05
影响因子：
11.1
通讯作者：
Altman, S
中科院分区：
文献类型：
--
作者：
GuerrierTakada, C;Salavati, R;Altman, S
含有编码称为外部引导序列（EGS）的小寡核糖核苷酸的合成基因的质粒已被引入带有抗生素抗性基因的大肠杆菌菌株中。 EGS指导RNase P切割从这些基因转录的mRNA，从而将耐药细胞的表型转化为药物敏感性，通过改变基因拷贝数或与不同靶位点互补的EGS数量来增加EGS与靶标mRNA的比率，从而提高了转换过程的效率，我们展示了耐药细菌培养物高效表型转化的通用方法。
Plasmids that contain synthetic genes coding for small oligoribonucleotides called external guide sequences (EGSs) have been introduced into strains of Escherichia coli harboring antibiotic resistance genes. The EGSs direct RNase P to cleave the mRNAs transcribed from these genes thereby converting the phenotype of drug-resistant cells to drug sensitivity, Increasing the EGS-to-target mRNA ratio by changing gene copy number or the number of EGSs complementary to different target sites enhances the efficiency of the conversion process, We demonstrate a general method for the efficient phenotypic conversion of drug-resistant bacterial cultures.