Chemopreventive effect of a xanthine oxidase inhibitor, 1'-acetoxychavicol acetate, on rat oral carcinogenesis
Chemopreventive effect of a xanthine oxidase inhibitor, 1'-acetoxychavicol acetate, on rat oral carcinogenesis
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DOI：
10.1111/j.1349-7006.1996.tb00229.x
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发表时间：
1996-04-01
期刊：
影响因子：
--
通讯作者：
Koshimizu, K
中科院分区：
文献类型：
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作者：
Ohnishi, M;Tanaka, T;Koshimizu, K
为探讨黄嘌呤氧化酶抑制剂1‘-乙酰氧基氨基酚醋酸酯(ACA)对4-硝基喹啉-1-氧化物(4-NQO)诱发口腔癌作用的影响，在F344雄性F344大鼠模型上，除ACA单独染毒组和未处理组外，所有大鼠饮水中加入4-NQO(20ppm)诱发口腔癌8周。4-NQO染毒前1周开始，饲喂含ACA 100ppm或500ppm的饲料10周，再给予不含ACA的基础饲料22周，其余两组分别给予100ppm或500ppm含ACA的饲料喂养22周。从4-NQO暴露停止后1周开始。在实验结束时(32周)，比较各组大鼠舌癌和癌前病变的发生率、舌组织中多胺水平以及用5-溴脱氧尿苷(BrdU)标记指数和银染核仁组织区蛋白(AgNORs)形态计量学分析评估细胞增殖活性。启动期和启动期后两种剂量的ACA均能显著降低舌癌的发生率(93%~100%，P
The effect of a xanthine oxidase inhibitor, 1'-acetoxychavicol acetate (ACA), on 4-nitroquinoline 1-oxide (4-NQO)-induced oral carcinogenesis was investigated in male F344 rats, All rats except those in the ACA-alone and untreated groups were given 4-NQO (20 ppm) in the drinking water for 8 weeks to induce oral cancer, Starting 1 week before the 4-NQO exposure, animals were fed diet containing 100 ppm or 500 ppm ACA for 10 weeks, followed by the basal diet without ACA for 22 weeks, Other groups were fed the diet containing ACA at 100 ppm or 500 ppm for 22 weeks, starting 1 week after the cessation of 4-NQO exposure. The remaining groups consisted of rats given 500 ppm ACA alone or untreated rats, At the termination of the experiment (32 weeks), the incidences of tongue neoplasms and preneoplastic lesions, polyamine levels in the tongue tissue, and cell proliferation activity estimated in terms of 5-bromodeoxyuridine (BrdU)-labeling index and by morphometric analysis of silver-stained nucleolar organizer regions' protein (AgNORs) were compared among the groups. Feeding of ACA at the two doses during initiation or postinitiation significantly decreased the development of tongue carcinoma (93-100% reduction, P