Effects of laying breeder hens dietary β-carotene, curcumin, allicin, and sodium butyrate supplementation on the jejunal microbiota and immune response of their offspring chicks.
Effects of laying breeder hens dietary β-carotene, curcumin, allicin, and sodium butyrate supplementation on the jejunal microbiota and immune response of their offspring chicks.
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DOI：
10.1016/j.psj.2020.03.065
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发表时间：
2020-08
期刊：
影响因子：
4.4
通讯作者：
Wu M
中科院分区：
文献类型：
--
作者：
Gong HZ;Lang WY;Lan HN;Fan YY;Wang TP;Chu QR;Wang JH;Li D;Zheng X;Wu M
我们的长期目标是通过母性效应改善雏鸡健康，减少家禽行业抗生素的使用。为了将空肠微生物与雏鸡不同的免疫水平和生长性能联系起来，本研究在不同组间比较的基础上，对空肠微生物、空肠炎症和免疫反应进行了研究。将新孵出的海兰雏鸡分为3组：雏鸡对照组（cCON）、环丙沙星乳酸处理组（Cipro）， cCON组和环丙沙星乳酸处理组（Cipro）从饲喂基础日粮的蛋鸡中孵出雏鸡；5周龄的β-胡萝卜素、姜黄素、大蒜素和丁酸钠补充组（cCCAB）从蛋鸡中孵出雏鸡。各组均饲喂相同饲粮4周；环丙沙星组患者连续饮水给予乳酸环丙沙星。实验结束时，结果表明，环丙沙星组空肠微生物在α和β多样性以及门和属水平的分类上发生了显著变化。经线性判别分析，组间共鉴定出67个显著富集（P < 0.05）的类群；cCCAB组显著富集厚壁菌门（P < 0.05）， Cipro组显著富集65个类群（P < 0.05），其中32个类群属于Cipro组的变形菌门（P < 0.05）。与cCON组相比，环丙沙星组和cCCAB组空肠内容物中脂多糖含量、空肠核因子κ b、肿瘤坏死因子α水平均升高（P < 0.05）。与cCON和cCCAB组相比，环丙环组空肠有明显的中性粒细胞浸润和IL-6 mRNA表达上调（均P < 0.05）。cCCAB组PSME3、PSME4基因表达较cCON、Cipro组上调（均P < 0.05）。综上所述，饲粮中添加环丙沙星可能通过微生物干扰引起的空肠炎症对雏鸡健康和生长造成潜在危害，蛋鸡饲粮中添加β-胡萝卜素、姜黄素、大蒜素和丁酸钠可通过宿主先天免疫选择微生物定植和微生物群诱导的适应性免疫相互作用增强子代空肠免疫。
Our long-term goal is to improve chick health and reduce the use of antibiotics in the poultry industry via maternal effects. To link jejunal microbes with chicks' different immune levels and growth performance in our previous research, this study investigated jejunal microbes, jejunal inflammation, and immune responses based on a comparison between different groups. Newly hatched Hy-Line chicks were allotted into 3 groups: a chick control group (cCON), a ciprofloxacin lactate treatment group (Cipro)—the chicks of the cCON and Cipro groups were hatched from laying breeder hens given a basal diet—and a 5-wk β-carotene, curcumin, allicin, and sodium butyrate supplementation group (cCCAB), wherein chicks hatched from laying breeder hens. All groups were fed the same diet for 4 wk; the Cipro group was given ciprofloxacin lactate in drinking water continuously. At the end of the experiment, the results demonstrated that the jejunal microbes of the Cipro group showed significant changes in alpha and beta diversity, and in taxonomy at phylum and genus levels. Statistically, a total of 67 significantly enriched (P < 0.05) taxa were identified between groups by linear discriminant analysis effect size; Firmicutes was significantly enriched (P < 0.05) in the cCCAB group, 65 taxa were significantly enriched (P < 0.05) in the Cipro group, and 32 of the 65 enriched (P < 0.05) taxa were in the Proteobacteria phylum of the Cipro group. Levels of lipopolysaccharide in jejunal content, and nuclear factor kappa-B, and tumor necrosis factor-α in jejunums of the Cipro and cCCAB groups were increased (all P < 0.05) compared to those in the cCON group. There was obvious neutrophil infiltration and upregulated (all P < 0.05) IL-6 mRNA in the Cipro group jejunums compared to the cCON and cCCAB groups. The expression of PSME3 and PSME4 genes was upregulated (all P < 0.05) in the cCCAB group compared to the cCON and Cipro groups. In conclusion, ciprofloxacin lactate administration led to potential hazards in health and growth in chicks via microbial disturbances-induced jejunal inflammation, and laying breeder hens dietary supplementation with β-carotene, curcumin, allicin, and sodium butyrate could enhance jejunal immunity of their offspring via the interaction between host innate immunity selected microbial colonization and microbiota educated adaptive immunity.
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DOI：
10.1007/0-387-30745-1_14
发表时间：
2006-01-01
期刊：
PROKARYOTES: A HANDBOOK ON THE BIOLOGY OF BACTERIA, VOL 5, THIRD EDITION
影响因子：
--
作者：
Green, Peter N.
通讯作者：
Green, Peter N.
DOI：
10.1073/pnas.0504978102
发表时间：
2005-08-02
影响因子：
11.1
作者：
Ley, RE;Bäckhed, F;Gordon, JI
通讯作者：
Gordon, JI
影响因子：
17.1
作者：
Scott NA;Andrusaite A;Andersen P;Lawson M;Alcon-Giner C;Leclaire C;Caim S;Le Gall G;Shaw T;Connolly JPR;Roe AJ;Wessel H;Bravo-Blas A;Thomson CA;Kästele V;Wang P;Peterson DA;Bancroft A;Li X;Grencis R;Mowat AM;Hall LJ;Travis MA;Milling SWF;Mann ER
通讯作者：
Mann ER
影响因子：
3.7
作者：
Dominguez, Jessica A.;Xie, Yan;Davidson, Nicholas O.
通讯作者：
Davidson, Nicholas O.
影响因子：
2.7
作者：
CHAO, A;MA, MC;YANG, MCK
通讯作者：
YANG, MCK