Quantitative proteomics analysis reveals proteins and pathways associated with anthocyanin accumulation in barley
Quantitative proteomics analysis reveals proteins and pathways associated with anthocyanin accumulation in barley
复制标题
定量蛋白质组学分析揭示了与大麦中花青素积累相关的蛋白质和途径
DOI：
10.1016/j.foodchem.2019.124973
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发表时间：
2019
期刊：
影响因子：
8.8
通讯作者：
Tashi Nyima
中科院分区：
文献类型：
--
作者：
Guoqiang Zhang;Wenhua Xue;Jie Dai;Qijun Xu;Yulin Wang;Hongjun Yuan;Kun Yang;Yiman Qi;Xingquan Zeng;Tashi Nyima
本研究的目的是利用定量蛋白质组学技术探讨紫大麦、蓝大麦和白色大麦花色素苷生物合成的内在机制。我们确定了蛋白质表达的差异和相关功能参与花青素的生物合成在紫色，蓝色和白色大麦（命名为H，M和L组，分别根据其花青素含量）使用TMT-液相色谱/质谱为基础的蛋白质组学方法。在H vs. L、H vs. M、L vs. M和H vs. L vs. M组中分别发现297、300、254和1421个差异表达蛋白（DEPs）。1421个DEPs中的6个蛋白质簇主要参与碳代谢、氨基酸和次生代谢产物的生物合成以及代谢途径。使用平行反应监测验证了几种蛋白质。氨基酸合成、碳代谢、代谢途径和苯丙烷类物质合成等相关蛋白质是造成3个大麦品种颜色差异的主要原因。
The aim of the present study was to explore the underlying mechanisms involved in anthocyanin biosynthesis in purple, blue, and white barley using quantitative proteomics analysis. We identified the differences in protein expression and related functions involved in anthocyanin biosynthesis in purple, blue, and white barley (named H, M, and L groups, respectively, based on their anthocyanin content) using TMT-liquid chromatography/mass spectroscopy-based proteomic methods. Totally, 297, 300, 254, and 1421 differentially expressed proteins (DEPs) were found in H vs. L, H vs. M, L vs. M, and H vs. L vs. M groups, respectively. Six clusters of proteins from the 1421 DEPs were mainly involved in carbon metabolism, amino acid and secondary metabolite biosynthesis, and metabolic pathways. Several proteins were validated using parallel reaction monitoring. The proteins involved in amino acid biosynthesis, carbon metabolism, metabolic pathways, and phenylpropanoid biosynthesis were responsible for the color differences in the three barley varieties.