Effect of lysyl oxidase (LOX) on corpus cavernous fibrosis caused by ischaemic priapism
Effect of lysyl oxidase (LOX) on corpus cavernous fibrosis caused by ischaemic priapism
复制标题
赖氨酰氧化酶(LOX)对缺血性阴茎异常勃起所致海绵体纤维化的影响
DOI：
10.1111/jcmm.13411
复制
发表时间：
2018-03-01
影响因子：
5.3
通讯作者：
Yuan, Jiuhong
中科院分区：
文献类型：
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作者：
Gao, Liang;Wu, Changjing;Yuan, Jiuhong
缺血性阴茎异常勃起（IP）引起的阴茎纤维化对患者的勃起功能有不利影响。本研究探讨赖氨酰氧化酶（LOX）在缺血性阴茎异常勃起（IP）后大鼠和人阴茎中的作用，以验证抗LOX药物在减轻IP所致大鼠阴茎纤维化和预防勃起功能障碍中的作用。72只大鼠随机分为6组：对照组、对照+ β-氨基丙腈（BAPN）组、9 h组、9 h + BAPN组、24 h组和24 h + BAPN组。β-氨基丙腈（BAPN），LOX的特异性抑制剂，在饮用水中给药。在1周和4周时，每组随机抽取一半大鼠进行实验。与对照组相比，IP 9和24 h组大鼠勃起功能明显下降，阴茎海绵体LOX表达明显上调。IP组大鼠阴茎海绵体成纤维细胞增多，平滑肌细胞/胶原比值降低，内皮连续性破坏，胶原异常沉积，纤维排列紊乱。各组间胶原I、III相对含量无明显差异。β-氨基丙腈（BAPN）能有效改善阴茎结构和勃起功能，促进恢复。本研究的数据表明LOX可能在IP后阴茎海绵体纤维化中起重要作用，抗LOX可能是IP患者的新靶点。
Penile fibrosis caused by ischemic priapism (IP) adversely affects patients' erectile function. We explored the role of lysyl oxidase (LOX) in rat and human penes after ischemic priapism (IP) to verify the effects of anti-LOX in relieving penile fibrosis and preventing erectile dysfunction caused by IP in rats. Seventy-two rats were randomly divided into six groups: control group, control + beta-aminopropionitrile (BAPN) group, 9 hrs group, 9 hrs + BAPN group, 24 hrs group, and 24 hrs + BAPN group. beta-aminopropionitrile (BAPN), a specific inhibitor of LOX, was administered in the drinking water. At 1 week and 4 weeks, half of the rats in each group were randomly selected for the experiment. Compared to the control group, the erectile function of IP rats was significantly decreased while the expression of LOX in the corpus cavernosum was significantly up-regulated in both 9 and 24 hrs group. Proliferated fibroblasts, decreased corpus cavernosum smooth muscle cells/collagen ratios, destroyed endothelial continuity, deposited abnormal collagen and disorganized fibers were observed in IP rats. The relative content of collage I and III was not obviously different among the groups. beta-aminopropionitrile (BAPN) could effectively improve the structure and erectile function of the penis, and enhance recovery. The data in this study suggests that LOX may play an important role in the fibrosis of corpus cavernosum after IP and anti-LOX may be a novel target for patients suffering with IP.