Human astrovirus C-terminal nsP1a protein is involved in RNA replication
Human astrovirus C-terminal nsP1a protein is involved in RNA replication
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DOI：
10.1016/j.virol.2004.12.023
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发表时间：
2005-03-01
期刊：
影响因子：
3.7
通讯作者：
Pintó, RM
中科院分区：
文献类型：
--
作者：
Guix, S;Caballero, S;Pintó, RM
人星状病毒非结构C-末端nsPla蛋白，其含有高变区（HVR）并与内质网和病毒RNA共定位，已被认为参与RNA复制过程。四种病毒不同，只有在其C-末端nsPla蛋白，对应于HVR衍生的基因型IV，V，VI和XII，都能够在CaCo-2细胞中复制，但显示其RNA复制和生长特性的差异。观察到两种总体复制模式：一侧为IV和V型，另一侧为VI和XII型。检测到的主要差异是在反基因组和亚基因组RNA的水平上，后者在IV型和V型中显著更高。因此，对来自胃肠炎儿童的粪便中的病毒RNA载量的定量显示，HVR衍生的基因型IV和V出现的数量显著更高。因此，可以得出结论，C-末端nsPla基因的变异性影响病毒复制表型。(C)2005年爱思唯尔公司All rights reserved.
Human astrovirus nonstructural C-terminal nsPla protein, which contains a hypervariable region (HVR) and colocalizes with the endoplasmic reticulum and viral RNA, has been suggested to be involved in the RNA replication process. Four viruses differing only in their C-terminal nsPla protein, corresponding to HVR-derived genotypes IV, V, VI, and XII, were all able to replicate in CaCo-2 cells but displayed differences in their RNA replication and growth properties. Two overall patterns of replication were observed: types IV and V on one side, and types VI and XII on the other. The main detected differences were on the levels of antigenomic and subgenomic RNAs, being the latter significantly higher in types IV and V. Accordingly, quantification of viral RNA load in feces from children with gastroenteritis showed that HVR-derived genotypes IV and V occur in significantly higher numbers. In consequence, it may be concluded that the variability of the C-terminal nsPla gene affects the virus replication phenotype. (C) 2005 Elsevier Inc. All rights reserved.