Functional characterization of electron-transferring flavoprotein and its dehydrogenase required for fungal development and plant infection by the rice blast fungus.
Functional characterization of electron-transferring flavoprotein and its dehydrogenase required for fungal development and plant infection by the rice blast fungus.
复制标题
稻瘟菌真菌发育和植物感染所需的电子传递黄素蛋白及其脱氢酶的功能表征
DOI：
10.1038/srep24911
复制
发表时间：
2016-04-26
影响因子：
4.6
通讯作者：
Lu G
中科院分区：
文献类型：
--
作者：
Li Y;Zhu J;Hu J;Meng X;Zhang Q;Zhu K;Chen X;Chen X;Li G;Wang Z;Lu G
电子转移黄蛋白（ETF）及其脱氢酶（ETFDH）是高度保守的电子载体，主要在线粒体脂肪酸β氧化中发挥作用。本文报道了稻瘟病菌（Magnaporthe）中ETF α和β亚基编码基因（ETFA和ETFB）和ETFDH编码基因（ETFDH）的鉴定和特征。研究表明，通过影响脂肪酸代谢，ETF和ETFDH突变导致严重的生长和分生孢子缺陷，这可以在很大程度上通过外源乙酸盐或碳酸盐来挽救。此外，虽然分生孢子萌发和附着胞形成似乎是正常的ETF和ETFDH突变体，大多数附着胞未能穿透寄主表皮由于低膨压。少数附着胞，成功地渗透受到严重限制，在入侵的增长，因此未能引起疾病。此外，ETF突变体etfb−诱导感染的宿主细胞中的ROS积累和外源抗氧化剂GSH加速突变体入侵生长而不增加穿透率。此外，突变体etfb−显示脂质体积累增加和ATP合成减少。因此，ETF和ETFDH在M.通过调节脂肪酸代谢、建立膨压和诱导宿主ROS积累来实现。
Electron-transferring flavoprotein (ETF) and its dehydrogenase (ETFDH) are highly conserved electron carriers which mainly function in mitochondrial fatty acid β oxidation. Here, we report the identification and characterization of ETF α and β subunit encoding genes (ETFA and ETFB) and ETFDH encoding gene (ETFDH) in the rice blast fungus Magnaporthe oryzae. It was demonstrated that, by impacting fatty acid metabolism, ETF and ETFDH mutations led to severe growth and conidiation defects, which could be largely rescued by exogenous acetate or carbonate. Furthermore, although conidium germination and appressorium formation appeared to be normal in ETF and ETFDH mutants, most appressoria failed to penetrate the host epidermis due to low turgor pressure. The few appressoria that succeeded in penetration were severely restricted in invasive growth and consequently failed to cause disease. Moreover, ETF mutant etfb− induced ROS accumulation in infected host cells and exogenous antioxidant GSH accelerated mutant invading growth without increasing the penetration rate. In addition, mutant etfb− displayed elevated lipid body accumulation and reduced ATP synthesis. Taken together, ETF and ETFDH play an important role in fungal development and plant infection in M. oryzae by regulation of fatty acid metabolism, turgor establishment and induction of host ROS accumulation.