CELL-LINES FROM CULICOIDES-VARIIPENNIS (DIPTERA, CERATOPOGONIDAE) SUPPORT REPLICATION OF BLUETONGUE VIRUS
CELL-LINES FROM CULICOIDES-VARIIPENNIS (DIPTERA, CERATOPOGONIDAE) SUPPORT REPLICATION OF BLUETONGUE VIRUS
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DOI：
10.1016/0022-2011(89)90123-7
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发表时间：
1989-11-01
影响因子：
3.4
通讯作者：
TABACHNICK, WJ
中科院分区：
文献类型：
--
作者：
WECHSLER, SJ;MCHOLLAND, LE;TABACHNICK, WJ
细胞系是由叮蠓库蠓 (Culicoides variipennis)（双翅目：Ceratopogonidae）两天大的胚胎发育而来。在北美，C. variipennis 是蓝舌病毒 (BTV) 的主要昆虫载体，蓝舌病毒是一种引起反刍动物疾病的环状病毒。在 Schneider 果蝇培养基中生长的 C. variipennis (CuVa) 细胞主要由成纤维细胞样细胞类型组成。 CuVa 细胞非常顽强。它们可以在很宽的温度和 pH 范围内生长，并适应在基本必需培养基中生长。 BTV 在 CuVu 细胞中在较宽的温度范围（19°C 至 37°C）内复制到高滴度（7.5-8.0 log10 50% 组织培养感染剂量/ml），而不诱导任何显着的细胞病变效应。在 25°C 下生长的 CuVa 细胞中获得了最高的 BTV 效价。和 32°C。来自 C. variipennis 的细胞可用于许多不同的研究。
Cell lines have been developed from 2-day-old embryos of the biting midge, Culicoides variipennis (Diptera: Ceratopogonidae). In North America C. variipennis is the primary insect vector of bluetongue virus (BTV), an orbivirus that causes disease of ruminants. The C. variipennis (CuVa) cells, grown in Schneider''s Drosophila medium, consist primarily of a fibroblast-like cell type. CuVa cells are very hardy. They can grow over a wide range of temperature and pH and adapt to growth in minimal essential medium. BTV replicates to high titer (7.5-8.0 log10 50% tissue culture infectious doses/ml) in CuVu cells over a wide range of temperatures (19.degree. to 37.degree.C) without inducing any significant cytopathic effects. The highest BTV titers were obtained in CuVa cells grown at 25.degree. and 32.degree.C. Cells from C. variipennis can be useful for many diverse investigations.