Kinetic analysis of a complete poxvirus transcriptome reveals an immediate-early class of genes
Kinetic analysis of a complete poxvirus transcriptome reveals an immediate-early class of genes
复制标题
DOI：
10.1073/pnas.0711573105
复制
发表时间：
2008-02-12
影响因子：
11.1
通讯作者：
Sette, Alessandro
中科院分区：
文献类型：
--
作者：
Assarsson, Erika;Greenbaum, Jason A.;Sette, Alessandro
牛痘病毒是正痘病毒的原型，是用于根除天花的疫苗，但其许多基因的表达谱仍然未知。使用基因组平铺阵列方法，我们同时测量了所有223个注释的牛痘病毒基因在感染过程中的表达水平，并确定了它们的动力学。对于这些基因中的95%，检测到显著的转录水平。最值得注意的是，通过基因表达谱对基因进行分类，发现35个基因表现出立即早期表达。虽然类似的动力学类已被描述为其他病毒家族，据我们所知，这是第一次证明其存在于正痘病毒。尽管表达水平高于其他三个动力学类的基因，但其中一半以上的功能仍然未知。此外，每个动力学类别内的基因在基因组中在空间上分组在一起。这种全基因组的转录改变了我们对正痘病毒如何调节基因表达的理解。
Vaccinia virus is the prototypic orthopoxvirus and was the vaccine used to eradicate smallpox, yet the expression profiles of many of its genes remain unknown. Using a genome tiling array approach, we simultaneously measured the expression levels of all 223 annotated vaccinia virus genes during infection and determined their kinetics. For 95% of these genes, significant transcript levels were detected. Most remarkably, classification of the genes by their expression profiles revealed 35 genes exhibiting immediate-early expression. Although a similar kinetic class has been described for other virus families, to our knowledge, this is the first demonstration of its existence in orthopoxviruses. Despite expression levels higher than for genes in the other three kinetic classes, the functions of more than half of these remain unknown. Additionally, genes within each kinetic class were spatially grouped together in the genome. This genome-wide picture of transcription alters our understanding of how orthopoxviruses regulate gene expression.