Activation of nonspecific killer cells by interleukin 2-containing supernatants.
Activation of nonspecific killer cells by interleukin 2-containing supernatants.
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含有白细胞介素 2 的上清液激活非特异性杀伤细胞。
DOI：
10.4049/jimmunol.131.4.1827
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发表时间：
1983
影响因子：
4.4
通讯作者：
M. Yu
中科院分区：
文献类型：
--
作者：
H. Teh;M. Yu
在无特异性抗原刺激的情况下，用含IL-2的培养上清液培养C57BL/6淋巴或脾细胞，以诱导非特异性杀伤细胞。这些上清液是用刀豆蛋白A刺激大鼠脾细胞或用佛波酯刺激T细胞淋巴瘤的变种EL4(H-2b)获得的。EL4上清液诱导非特异性杀伤细胞的能力可被依赖于白细胞介素2的T细胞系或刀豆蛋白A刺激的脾细胞所消除，但不能被内毒素刺激的脾细胞或不产生白细胞介素2的EL4细胞所消除。EL4上清液中部分纯化的IL-2也可支持非特异性杀伤细胞的诱导。白细胞介素2诱导的细胞溶解细胞对伽马射线敏感，其D值为120rad。诱导的非特异性杀伤细胞可能是细胞毒性T淋巴细胞；大多数前体细胞和效应细胞携带Thy-1同种异体抗原标记。这些非特异性杀伤细胞杀死了广泛的靶细胞，包括刀豆蛋白A和脂多糖诱导的同基因或异基因小鼠的脾母细胞，同基因肿瘤和克隆的同种异体细胞毒T细胞系。C57BL/6淋巴结和脾细胞中非特异性杀伤细胞前体细胞的频率分别为1/7000和1/12000。克隆分析显示，这些非特异性杀伤细胞在其靶细胞特异性方面表现出异质性。含有IL-2的培养上清液诱导的非特异性杀伤细胞部分依赖于尼龙羊毛贴壁细胞；在抗原刺激培养中，尼龙羊毛非贴壁淋巴结反应细胞被尼龙羊毛非贴壁同种异体脾刺激细胞刺激，再经抗Thy-1抗体和补体处理，获得最特异的杀伤细胞。这些发现与白介素2补充培养中产生的细胞毒性T淋巴细胞的频率和精细特异性的相关性进行了讨论。
In the absence of specific antigen stimulation, nonspecific killer cells were induced by culturing C57BL/6 lymph node or spleen cells with interleukin 2-containing supernatants. These supernatants were obtained from stimulation of either rat spleen cells with concanavalin A or a variant of the T cell lymphoma, EL4 (H-2b) with phorbol myristic acetate. The ability of the EL4 supernatant to induce nonspecific killer cells was abrogated by absorption with an interleukin 2-dependent T cell line or by concanavalin A-stimulated spleen cell blasts, but not by lipopolysaccharide-stimulated spleen cell blasts or by a non-interleukin 2-producing EL4 line. Partially purified interleukin 2 from EL4 supernatants could also support nonspecific killer cell induction. The induction of cytolytic cells by interleukin 2 is sensitive to gamma-irradiation and has a D omicron of 120 rad. The nonspecific killer cells induced are likely cytotoxic T lymphocytes; the majority of the precursor and effector cells bear the Thy-1 alloantigen marker. These nonspecific killer cells killed a broad spectrum of target cells, including concanavalin A- and lipopolysaccharide-induced splenic blasts of syngeneic or allogeneic mice, a syngeneic tumor, and a cloned allogeneic cytotoxic T cell line. The frequency of precursors for nonspecific killer cells in C57BL/6 lymph node and spleen cells are 1/7000 and 1/12,000, respectively. Clonal analyses revealed that these nonspecific killers exhibit heterogeneity with respect to their target cell specificities. The induction of nonspecific killers by interleukin 2-containing supernatants is partially dependent on nylon wool-adherent cells; in antigen-stimulated cultures the most specific killer cells were obtained from cultures in which nylon wool-nonadherent lymph node responder cells were stimulated with nylon wool-nonadherent allogeneic splenic stimulator cells that were treated with anti-Thy-1 antibody and complement. The relevance of these findings with respect to the frequencies and fine specificities of cytotoxic T lymphocytes generated in interleukin 2-supplemented cultures is discussed.