Resveratrol attenuates inflammatory hyperalgesia by inhibiting glial activation in mice spinal cords
Resveratrol attenuates inflammatory hyperalgesia by inhibiting glial activation in mice spinal cords
复制标题
白藜芦醇通过抑制小鼠脊髓神经胶质细胞活化减轻炎症性痛觉过敏
DOI：
10.3892/mmr.2016.5027
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Ying‑Ying Chen
中科院分区：
文献类型：
--
作者：
Lin‑Lin Wang;Dong‑Ling Shi;Hui‑Yao Gu;Ming‑Zhi Zheng;Jue Hu;Xing‑Hui Song;Yue‑Liang Shen;Ying‑Ying Chen
本研究旨在探讨白藜芦醇对炎症性疼痛的影响。小鼠腹腔注射脂多糖（LPS）连续5天，诱导亚急性全身炎症。在最后一次LPS注射后进行乙酸诱导的扭体试验和甩尾试验。采用免疫组织化学分析或蛋白质印迹法检测胶质细胞酸性蛋白（GFAP;星形胶质细胞特异性活化标志物）、离子钙结合衔接分子1（Iba-1;小胶质细胞特异性活化标志物）和沉默调节蛋白1（SIRT 1）蛋白表达水平。给予LPS 5天后，扭体次数增加，甩尾潜伏期缩短。白藜芦醇（10或20 mg/kg）部分抑制LPS诱导的痛觉过敏，并阻止LPS诱导的肿瘤坏死因子-α和白细胞介素6水平的升高。LPS注射降低了SIRT 1蛋白的表达，并增加了脊髓中GFAP阳性和Iba-1阳性细胞的数量。白藜芦醇增加了SIRT 1蛋白的表达水平，并减少了LPS处理小鼠中GFAP阳性和Iba-1阳性细胞的数量。白藜芦醇的保护作用部分被选择性SIRT 1抑制剂EX-257阻断。本研究的结果表明，亚急性处理与LPS诱导神经胶质细胞的激活和痛觉过敏。白藜芦醇被证明可以抑制神经胶质细胞的活化，并以SIRT 1依赖的方式减轻炎症性痛觉过敏。
The present study aimed to investigate the effect of resveratrol on inflammatory pain. Mice were injected intraperitoneally with lipopolysaccharide (LPS) for 5 consecutive days to induce subacute systemic inflammation. Acetic acid‑induced writhing tests and tail‑flick tests were performed following the final LPS injection. Glial fibrillary acidic protein (GFAP; an astrocyte‑specific activation marker), ionized calcium binding adapter molecule 1 (Iba‑1; a microglia‑specific activation marker) and sirtuin 1 (SIRT1) protein expression levels were detected using immunohistochemistry analysis or western blotting. Following administration of LPS for 5 days, the number of writhes increased and the tail‑flick latency decreased. Resveratrol (10 or 20 mg/kg) partly inhibited LPS‑induced hyperalgesia and prevented the increase in tumor necrosis factor‑α and interleukin 6 levels induced by LPS. LPS injection reduced the SIRT1 protein expression and increased the number of GFAP‑positive and Iba‑1‑positive cells in the spinal cord. Resveratrol increased the SIRT1 protein expression levels and decreased the number of GFAP‑positive and Iba‑1‑positive cells in LPS‑treated mice. The protective effect of resveratrol was partly blocked by a selective SIRT1 inhibitor, EX‑257. Results from the present study suggest that subacute treatment with LPS induced the activation of glial cells and hyperalgesia. Resveratrol was demonstrated to inhibit the activation of glial cells and attenuate inflammatory hyperalgesia in a SIRT1‑dependent manner.