Utilization of monocrotophos as phosphorus source by Pseudomonas aeruginosa F10B and Clavibacter michiganense subsp. insidiosum SBL 11.
Utilization of monocrotophos as phosphorus source by Pseudomonas aeruginosa F10B and Clavibacter michiganense subsp. insidiosum SBL 11.
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DOI：
10.1139/w03-013
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发表时间：
2003-02
影响因子：
2.8
通讯作者：
S. Singh;D. Singh
中科院分区：
文献类型：
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作者：
S. Singh;D. Singh
从土壤中分离出的微生物将有机磷杀虫剂一氯磷(二甲基（E)-1-甲基-2-（甲氨酰基）乙烯磷酸盐或MCP）作为唯一的磷源。没有一个分离物可以利用MCP作为碳的唯一来源。两个潜在的微生物分离株，铜绿假单胞菌F10B和密歇根锁杆菌亚种。sidiosum sbl11可以利用MCP作为磷的唯一来源。铜绿假单胞菌F10B潜伏期为4 h，密歇根假单胞菌F10B潜伏期为4 h。sidiosum SBL 11，在MCP存在下培养8 h。在含有MCP的培养基中，两种菌株的生长时间均有所延长。铜绿假单胞菌F10B在MCP培养基中为2.15 h，在基础培养基中为1.29 h；sidiosum SBL 11在MCP培养基中的生长时间由基础培养基的1.28 h增加到3.4 h。在37℃的摇瓶培养条件下，这两株菌株在24 h内对MCP的降解率分别为98.9%和86.9%，对纯MCP的降解率分别为79%和80%，正常生长所需的MCP的最佳浓度为500 ppm。在底物偏好研究中，Tris-p-nitrophenyl phosphate是最受欢迎的底物，其次是对氧磷。负责MCP分解的酶是磷酸三酯酶，它定位于被破坏细胞的膜结合部分。铜绿假单胞菌F10B中产生磷酸三酯酶（opd）的基因是质粒携带的。
Monocrotophos (dimethyl (E)-1-methyl-2-(methylcarbamoyl) vinyl phosphate, or MCP), an organophosphorus insecticide, was used as a sole phosphorus source by the microorganisms isolated from the soil. None of the isolates could utilize MCP as a sole source of carbon. Two of the potential microbial isolates, Pseudomonas aeruginosa F10B and Clavibacter michiganense subsp. insidiosum SBL 11, could utilize MCP as a sole source of phosphorus. Pseudomonas aeruginosa F10B showed a lag phase of 4 h, while in the case of C. michiganense subsp. insidiosum SBL 11, it was 8 h when cultured in the presence of MCP. The generation time for both strains was increased in the medium containing MCP. It was 2.15 h for P. aeruginosa F10B in MCP medium as compared with 1.29 h in basal medium, while in case of C. michiganense subsp. insidiosum SBL 11 it was increased to 3.4 h in MCP medium as compared with 1.28 h in basal medium. These two strains were able to degrade technical MCP in shake-flask culture up to 98.9 and 86.9%, respectively, and pure MCP up to 79 and 80%, respectively, within 24 h at 37 degrees C. The optimal concentration of MCP required for the normal growth was 500 ppm. In the substrate preference study, Tris-p-nitrophenyl phosphate was the most preferred substrate followed by paraoxon. The enzyme responsible for the break down of MCP was phosphotriesterase, which was localized on the membrane-bound fraction of the disrupted cells. The gene responsible for the production of phosphotriesterase (opd) in P. aeruginosa F10B was plasmid-borne.