Quality control during the conventional analysis of semen, an essential exercise.
Quality control during the conventional analysis of semen, an essential exercise.
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精液常规分析过程中的质量控制是一项重要的工作。
DOI：
10.1002/j.1939-4640.1989.tb00124.x
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发表时间：
1989
影响因子：
--
通讯作者：
I. D. Cooke
中科院分区：
文献类型：
--
作者：
B. Dunphy;R. Kay;C. Barratt;I. D. Cooke
通过世界卫生组织（世卫组织）最近提倡的方法评估精子活力的技术人员内部和内部差异进行了研究。在估计精子浓度的内部和技术人员之间的可变性和在评估精子形态的技术人员内部的可变性也进行了检查。评估精子活力的技术人员内部差异似乎与观察者的自然能力和/或训练有关。虽然一般来说，技术人员内部的可变性很低，但在评估相同精液样本时，观察者之间存在显著的临床显著差异。在精子浓度的评估中，观察者之间没有显著差异，技术人员内部的可变性很低。在评估精子形态时，技术人员内部的差异可能很大（超过20%形态理想精子的水平）。应招聘具有观察能力的技术人员。对于任何计划将精液参数与生育结果联系起来的中心来说，质量控制似乎是一项必不可少的工作。
Intra- and inter-technician variability in assessing sperm motility by the methods recently advocated by the World Health Organization (WHO) were studied. The intra- and inter-technician variability in estimating sperm concentration and the intra-technician variability in assessing sperm morphology were also examined. Intra-technician variability in assessing sperm motility appeared to be related to the natural ability and/or training of the observer. Although in general the intra-technician variability was low, there were marked and clinically significant differences between observers when assessing the same semen sample. There was no significant difference between observers in the assessment of sperm concentration, and intra-technician variability was low. When assessing sperm morphology, the intra-technician variability was potentially large (above a level of 20% morphologically ideal spermatozoa). Technicians should be recruited who have natural ability as observers. Quality control appears to be an essential exercise for any center that plans to relate semen parameters to fertility outcome.