Immunolocalization of a human cementoblastoma-conditioned medium-derived protein
Immunolocalization of a human cementoblastoma-conditioned medium-derived protein
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DOI：
10.1177/154405910208100808
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发表时间：
2002-08-01
影响因子：
7.6
通讯作者：
Pitaru, S
中科院分区：
文献类型：
--
作者：
Arzate, H;Jiménez-García, LF;Pitaru, S
由于目前还没有特异性的牙骨质标记物，因此对调节牙骨质形成过程的分子机制知之甚少。为了研究成牙骨质细胞瘤条件培养基衍生蛋白（CP）是否可用作牙骨质生物标记物，我们研究了其在人牙周组织、人牙周韧带、牙槽骨和成牙骨质细胞瘤衍生细胞中的表达和分布。在人牙周组织中，在整个牙骨质样期的无细胞和细胞牙骨质，成牙骨质细胞，牙骨质细胞，细胞位于人牙槽骨的骨内膜空间，并在细胞中的牙周韧带位于血管附近的免疫反应性抗CP观察。免疫纯化CP促进细胞附着在人牙周膜，牙槽骨衍生细胞，牙龈成纤维细胞。牛牙骨质附着蛋白（CAP）单克隆抗体与CP发生交叉反应。这些发现表明，CP识别潜在的成牙骨质细胞祖细胞，是免疫相关的CAP物种，并作为牙骨质的生物学标记。
Little is known about the molecular mechanisms that regulate the cementogenesis process, because specific cementum markers are not yet available. To investigate whether a cementoblastoma-conditioned medium-derived protein (CP) could be useful as a cementum biological marker, we studied its expression and distribution in human periodontal tissues, human periodontal ligament, alveolar bone, and cementoblastoma-derived cells. In human periodontal tissues, immunoreactivity to anti-CP was observed throughout the cementoid phase of acellular and cellular cementum, cementoblasts, cementocytes, cells located in the endosteal spaces of human alveolar bone, and in cells in the periodontal ligament located near the blood vessels. Immunopurified CP promoted cell attachment on human periodontal ligament, alveolar bone-derived cells, and gingival fibroblasts. A monoclonal antibody against bovine cementum attachment protein (CAP) cross-reacted with CP. These findings indicate that CP identifies potential cementoblast progenitor cells, is immunologically related to CAP species, and serves as a biological marker for cementum.