Hyperadrenocorticism and food restriction-induced life extension in the rat: evidence for divergent regulation of pituitary proopiomelanocortin RNA and adrenocorticotropic hormone biosynthesis.
Hyperadrenocorticism and food restriction-induced life extension in the rat: evidence for divergent regulation of pituitary proopiomelanocortin RNA and adrenocorticotropic hormone biosynthesis.
复制标题
肾上腺皮质机能亢进和食物限制引起的大鼠寿命延长：垂体阿黑皮质素原 RNA 和促肾上腺皮质激素生物合成的不同调节的证据。
DOI：
10.1093/gerona/50a.5.b288
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Nelson,JF
中科院分区：
文献类型：
--
作者：
Han,ES;Levin,N;Bengani,N;Roberts,JL;Suh,Y;Karelus,K;Nelson,JF
限食（FR）引起的血浆皮质酮(B)日升高可能与延长寿命有关。我们研究了FR是否会改变血浆和垂体前叶（AP）中促肾上腺皮质激素（ACTH）和促黑素原（POMC） mRNA水平，因为这些分子都能调节被b抑制的andean。对3个月大的雄性Fischer 344大鼠进行了测量，这些大鼠从6周龄开始被随意喂食（AL）或FR （60% AL卡路里）。FR大鼠的血浆B在PM样品中高出2倍，但在AM样品中没有差异。相比之下，FR大鼠的PM样品和AL大鼠的血浆ACTH没有差异，FR大鼠的AM样品低20% （p < 0.05）。AM和PM样品中FR大鼠ACTH AP含量均降低50% （p < 0.01）。相比之下，FR大鼠POMC mRNA、初级转录物和加工中间体的AP含量未降低，而POMC初级转录物的PM含量升高（p < 0.05）。FR大鼠垂体和血浆ACTH的降低可能是其血浆B水平升高的结果。该研究还表明，除了ACTH升高外，其他因素也可解释fr诱导的肾上腺皮质亢进。这些结果也表明，FR大鼠的POMC mRNA和ACTH生物合成受到差异调节。
The increased diurnal elevation of plasma corticosterone (B) induced by food restriction (FR) may play a role in the life span extension ofFR. We investigated whether FR alters adrenocorticotropic hormone (ACTH) and proopiomelanocortin (POMC) mRNA levels in plasma and anterior pituitary (AP), since these molecules both regulate andean be suppressed byB. Measurements were made in 3-month-old male Fischer 344 rats that had been fed ad libitum (AL) or FR (60% ofAL calories) since 6 weeks of age. Plasma B was 2-fold higher in FR rats in the PM samples, but did not differ in AM samples. By contrast, plasma ACTH did not differ in the PM samples of FR and AL rats and was 20% lower in AM samples (p < .05) of FR rats. AP content of ACTH was 50% lower in FR rats in both AM and PM samples (p < .01). In contrast, AP contents of POMC mRNA, primary transcript, and processing intermediate were not reduced in FR rats, and PM content of POMC primary transcript was elevated in FR rats (p < .05). The reduced pituitary and plasma ACTH of FR rats may be the consequence of their elevated plasma B levels. This study also suggests that factors other than elevated ACTH account for FR-induced hyperadrenocorticism. These results also indicate that POMC mRNA and ACTH biosyntheses are differentially regulated in FR rats.