Changes in duodenal and nephritic Ca and P absorption in hens during different egg-laying periods.
Changes in duodenal and nephritic Ca and P absorption in hens during different egg-laying periods.
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不同产蛋期母鸡十二指肠及肾组织钙、磷吸收变化
DOI：
10.1016/j.heliyon.2021.e06081
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发表时间：
2021-01
期刊：
影响因子：
4
通讯作者：
Wu G
中科院分区：
文献类型：
--
作者：
San J;Zhang Z;Bu S;Zhang M;Hu J;Yang J;Wu G
产蛋期钙磷代谢紊乱可导致产蛋量下降、蛋壳质量下降，甚至引起母鸡骨骼问题;然而，关于十二指肠和肾脏钙磷吸收能力的研究却知之甚少。本研究测定了不同产蛋期蛋鸡血清钙、磷代谢指标及十二指肠和肾脏钙、磷转运蛋白基因的表达水平。Ca、25-（OH）-VD 3和1，25-（OH）2-VD 3含量在产蛋高峰期（43周龄）和产蛋后期（72周龄）较产蛋前期（23周龄）增加，而Pi含量在3个产蛋期间无差异。产蛋高峰期和产蛋后期，十二指肠VDR和CaBP-D28 k mRNA的表达显著高于产蛋前期，而NPt 2b mRNA的表达显著低于产蛋前期。此外，与产蛋前期相比，产蛋高峰期和产蛋后期肾脏CaBP-D28 k、PMCA 1b和FGFR 1 mRNA的表达显著升高，而NPt 2a和Cyp 24 a1 mRNA的表达显著降低。总之，本研究表明，在产蛋高峰期和产蛋后期，十二指肠和肾脏钙吸收增加可能与VD-VDR途径有关，而在所有三个产蛋阶段，尽管血清磷水平相对稳定，但磷吸收减少可能与骨质溶解有关。钙磷吸收;转运蛋白表达;十二指肠;肾脏;不同产蛋期;母鸡。
Ca and P metabolic disorders during the egg-laying period can reduce egg production, impair eggshell quality, and even cause bone problems in hens; however, little is known regarding the capacity of duodenal and nephritic Ca and P absorption. Here, the levels of serum Ca and P metabolic indices and the expression of duodenal and renal Ca and P transporter genes were measured in hens at different egg-laying stages. The Ca, 25-(OH)-VD3, and 1,25-(OH)2-VD3 content increased during the peak (43 weeks of age) and late (72 weeks of age) egg-laying periods compared to that during the early (23 weeks of age) egg-laying period; however, there were no differences in Pi levels among the three egg-laying periods. Moreover, duodenal VDR and CaBP-D28k mRNA expression was markedly higher but NPt2b mRNA expression was markedly lower during the peak and late egg-laying periods than during the early egg-laying period. Furthermore, nephritic CaBP-D28k, PMCA1b, and FGFR1 mRNA expression was markedly higher but NPt2a and Cyp24a1 mRNA expression was markedly lower during the peak and late egg-laying periods than during the early egg-laying period. In conclusion, the present study indicated that the increased duodenal and nephritic Ca absorption during the peak and late egg-laying periods may be associated with the VD–VDR pathway, while the decreased P absorption despite relatively stable serum P levels in all three egg-laying stages may associated with osteolysis. Ca and P absorption; Transporter expression; Duodenum; Kidney; Different laying stages; Hens.